|Table of Contents|

NCAPD3 Promotes the Occurrence and Development ofProstate Cancer Through EZH2(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2021年04期
Page:
53-59
Research Field:
·生物学·
Publishing date:

Info

Title:
NCAPD3 Promotes the Occurrence and Development ofProstate Cancer Through EZH2
Author(s):
Liu Qianmei; Ke Min; He Xinyuan; Jing Zuolei; Liu Ping
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Nanjing 210023,China
Keywords:
Prostate cancer; NCAPD3; EZH2; AR
PACS:
R36
DOI:
10.3969/j.issn.1001-4616.2021.04.007
Abstract:
NCAPD3(non-SMC condensin II complex subunit D3)is one of the non-SMC regulatory subunits of Condensin II,which is responsible for the condensation and segregation of chromosomes during mitosis and plays a role in promoting cancer in different cancers. This study explored that NCAPD3 promotes the occurrence and development of prostate cancer by regulating the expression of EZH2(enhancer of Zeste 2 polycomb repressive complex 2 subunit). Firstly,the differential expressions of NCAPD3 and EZH2 between normal and tumor tissues were analyzed through the database. Then,the levels of NCAPD3 and EZH2 in prostate cancer were detected by Western blot and IHC,and found that both NCAPD3 and EZH2 were highly expressed in prostate cancer cells and clinical tissues. After overexpressing or knocking down NCAPD3 in cells,the protein and mRNA levels of EZH2 increased or decreased accordingly,indicating that NCAPD3 regulates the expression of EZH2 at the transcriptional level. Finally,by overexpressing or knocking down AR(androgen receptor),it was found that the levels of NCAPD3 and EZH2 increased or decreased correspondingly,suggesting that there is an AR-NCAPD3-EZH2 signaling pathway in prostate cancer.

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Last Update: 2021-12-15