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Perithecia of Promoting Culture and Identification ofEndophytic Diaporthe sojae(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2021年04期
Page:
60-68
Research Field:
·生物学·
Publishing date:

Info

Title:
Perithecia of Promoting Culture and Identification ofEndophytic Diaporthe sojae
Author(s):
Dou Mingzhu; Pu Shunchang; Yan Shuzheng; Chen Shuanglin
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
Glycine soja; endophytic fungi; perithecia; culture method
PACS:
Q945
DOI:
10.3969/j.issn.1001-4616.2021.04.008
Abstract:
In this paper,a method for increasing the quantity and promoting the maturity of perithecia of an endophytic Diaporthe species from Glycine soja is introduced and described. The morphological characteristics and molecular sequence of the isolates and cultures of endogenous mesial shell fungi from Glycine soja are identified. Potato dextrose agar(PDA)and water agar(WA)were used to inoculate the isolated Glycine soja endogenous mesocapsid strain cake,while sterilized soybean stalks and extinct bacteria were placed in parallel on both sides of the inoculated fungal strain pine needles,which are used to induce to produce perithecia on sterilized soybean stalks and sterilized pine needles,and then cultured at 12 h light vs. 12 h dark and 25 ℃,perithecia were continuously produced in the medium with sterilized soybean stalks and sterilized pine needles,and the number was significantly greater than that in the medium of PDA. The isolate was identified as Diaporthe sojae by its morphological characteristics and molecular sequence. This species was previously reported to occur on Glycine max,causing stem blight,and it was the first record on Glycine soja as endophytic fungi. The results of phylogenetic analysis of ITS sequences support Diaporthe kochmanii is an idea of naming a different name for Diaporthe sojae.

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Last Update: 2021-12-15