|Table of Contents|

cDNA Cloning,Characterization and Sequence Analyses of14-3-3ζ Gene in Eriocheir sinensis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2021年04期
Page:
69-75
Research Field:
·生态学·
Publishing date:

Info

Title:
cDNA Cloning,Characterization and Sequence Analyses of14-3-3ζ Gene in Eriocheir sinensis
Author(s):
Xiao Qi1; Wu Yanbing1; Chen Lian2; Li Peng1
(1.School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210023,China)(2.College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)
Keywords:
Eriocheir sinensis; 14-3-3ζ; cDNA; sequence analyses
PACS:
Q958
DOI:
10.3969/j.issn.1001-4616.2021.04.009
Abstract:
14-3-3 proteins can bind to different proteins to participate in a variety of phosphorylation regulatory signaling pathways,and play an important role in biological metabolism,signal transduction,cell cycle control,apoptosis,protein transport,transcription,stress response and other aspects. The full-length cDNA of 14-3-3ζ gene from the Chinese mitten crab Eriocheir sinensis was cloned and identified using SMART RACE technique. It is consisted of 1 081 nucleotides with an open-reading frame of 744 bp,which encoding 247 amino acids(28.0 kDa)and containing 14-3-3 domain. Sequence alignment,phylogenetic tree construction and some bioinformatics analyses revealed that 14-3-3ζ is non-secretory and no-transmembrane protein,which is probable located in cytoplasm. The molecular weight of 14-3-3ζ is similar than that of others 14-3-3ζ isoforms. The sequence of 14-3-3ζ in E.sinensis is highly homologous to that in the reported crustaceans such as Penaeus vannamei and Scylla paramamosain. Moreover,we observed a strong signature of purifying selection between the comparison of orthologous 14-3-3ζ gene pairs of E.sinensis. The results suggest a conservative function across crustaceans.

References:

[1] MOORE B W,PEREZ V J,GEHRING M. Assay and regional distribution of a soluble protein characteristic of the nervous system[J]. Journal of neurochemistry,1968,15(4):265-272.
[2]周颖,李冰樱,李学宝. 14-3-3蛋白对植物发育的调控作用[J]. 植物学报,2012,47(1):55-64.
[3]刘漫,王月华,杜冠华. 14-3-3蛋白生物学特征及相关药物发现[J]. 中国新药杂志,2020,29(14):1591-1595.
[4]YAFFE M B. How do 14-3-3 proteins work?— Gatekeeper phosphorylation and the molecular anvil hypothesis[J]. FEBS letters,2002,513(1):53-57.
[5]MUSLIN A J. Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine[J]. Cell,1996,84(6):889-897.
[6]ICHIMURA T,ISOBE T,OKUYAMA T,et al. Brain 14-3-3 protein is an activator protein that activates tryptophan 5-monooxygenase and tyrosine 3-monooxygenase in the presence of Ca2+,calmodulin-dependent protein kinase II[J]. FEBS letters,1987,219(1):79-82.
[7]HEUSDEN G P H V. 14-3-3 proteins:Insights from genome-wide studies in yeast[J]. Genomics,2009,94(5):287-293.
[8]AITKEN A. 14-3-3 proteins:a historic overview[J]. Seminars in cancer biology,2006,16(3):162-172.
[9]DARLING D L,YINGLING J,WYNSHAW B A. Role of 14-3-3 proteins in Eukaryotic signaling and development[J]. Current topics in developmental biology,2005,68(4):281-315.
[10]DALAL S N,YAFFE M B,DECAPRIO J A. 14-3-3 Family members act coordinately to regulate mitotic progression[J]. Cell cycle,2004,3(5):670-675.
[11]LEE I N,CHEN C H,SHEU J C,et al. Identification of human hepatocellular carcinoma-related biomarkers by two-dimensional difference gel electrophoresis and mass spectrometry[J]. Journal of proteome research,2005,4(6):2062-2069.
[12]TELLES E,HOSING A S,KUNDU S T,et al. A novel pocket in 14-3-3ε is required to mediate specific complex formation with cdc25C and to inhibit cell cycle progression upon activation of checkpoint pathways[J]. Experimental cell research,2009,315(8):1448-1457.
[13]LI M,REN L,XU B,et al. Genome-wide identification,phylogeny,and expression analyses of the 14-3-3 family reveal their involvement in the development,ripening,and abiotic stress response in banana[J]. Frontiers in plant science,2016,7:1442.
[14]KILANI R T,MEDINA A,AITKEN A,et al. Identification of different isoforms of 14-3-3 protein family in human dermal and epidermal layers[J]. Molecular & cellular biochemistry,2008,314(1-2):161-169.
[15]LI J,XU H,WANG Q,et al. 14-3-3ζ promotes gliomas cells invasion by regulating Snail through the PI3K/AKT signaling[J]. Cancer medicine,2019,8(2):783-794.
[16]唐裕福,张怡冰,冯晓东,等. 14-3-3蛋白在人类疾病中的研究进展[J]. 世界华人消化杂志,2017,25(6):509-520.
[17]褚明辉,汪发九,赵国芳. 14-3-3zeta在人类常见恶性肿瘤中的研究进展[J]. 现代实用医学,2020,32(11):1433-1436.
[18]LU Q,WU S,ZHEN H,et al. 14-3-3 α and 14-3-3 ζ contribute to immune responses in planarian Dugesia japonica[J]. Gene,2017,615:25-34.
[19]TRUJILLO O A,CáZARES R F E,DEL ANGEL R M,et al. Participation of 14-3-3ε and 14-3-3ζ proteins in the phagocytosis,component of cellular immune response,in Aedes mosquito cell lines[J]. Parasites & vectors,2017,10(1):362.
[20]王晓伟,高洁,张子平,等. 拟穴青蟹14-3-3ζ基因cDNA的克隆和表达分析[J]. 上海海洋大学学报,2013,22(4):496-501.
[21]KATOH K,STANDLEY D M. MAFFT multiple sequence alignment software version 7:improvements in performance and usability[J]. Molecular biology & evolution,2013:30(4),772-780.
[22]SUDHIR K,GLEN S,KOICHIRO T. MEGA7:molecular evolutionary genetics analysis version 7.0 for gigger datasets[J]. Molecular biology & evolution,2016,33(7):1870-1874.
[23]YANG Z. PAML4:phylogenetic analysis by maximum likelihood[J]. Molecular biology & evolution,2007,24(8):1586-1591.
[24]BURLAND T G. DNASTAR’s Lasergene sequence analysis software[J]. Methods of molecular biology,2000,132:71-91.
[25]FUKAI M,KOBAYASHI N,ISHIKAWA T,et al. 14-3-3ζ-mediated stimulation of oxidative phosphorylation exacerbates oxidative damage under hypothermic oxygenated conditions in human renal tubular cells(HK-2)[J]. Transplantation proceedings,2016,48(4):1288-1291.
[26]JIA Y,LI H Y,WANG J,et al. Phosphorylation of 14-3-3ζ links YAP transcriptional activation to hypoxic glycolysis for tumorigenesis[J]. Oncogenesis,2019,8(5):31.
[27]MESSARITOU G,GRAMMENOUDI S,SKOULAKIS E M. Dimerization is essential for 14-3-3zeta stability and function in vivo[J]. Journal of biological chemistry,2010,285(3):1692-700.
[28]潘伟男,陈锋,封芬,等. 14-3-3蛋白的研究进展[J]. 国际病理科学与临床杂志,2007,27(3):262-265.
[29]LIU D,BIENKOWSKA J,PETOSA C,et al. Crystal structure of the zeta isoform of the 14-3-3 protein[J]. Nature,1995,376(6536):191-194.

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Last Update: 2021-12-15