|Table of Contents|

Marsupenaeus japonicus shrimp miR-34 Target Gene Prediction and Bioinformatics Analysis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2022年02期
Page:
52-62
Research Field:
·生物学·
Publishing date:

Info

Title:
Marsupenaeus japonicus shrimp miR-34 Target Gene Prediction and Bioinformatics Analysis
Author(s):
Li Fen; Liu Xinhe; Gao Mingyue; Xu Chen; Xu Junying; Shi Yinghua; Cui Yalei
(College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450046,China)
Keywords:
Marsupenaeus japonicus shrimp; mja-miR-34; target genes; bioinformatic analysis
PACS:
S9
DOI:
10.3969/j.issn.1001-4616.2022.02.007
Abstract:
Previous study has shown that miR-34(mja-miR-34)of Marsupenaeus japonicus shrimp is involved in the regulation of white spot syndrome virus(WSSV)infection,but the host genes regulated by mja-miR-34 have not yet been elucidated. In this study,the sequences of miR-34 in 17 species were firstly compared,and the target genes of shrimp regulated by miR-34 were predicted by TargetScan 5.1 and miRanda. The results showed that miR-34 was highly conserved during species evolution; mja-miR-34 could target 242 host-encoded genes and was differentially expressed in different time periods of virus infection; the target genes of mja-miR-34 were involved in the regulation of multiple cellular process of cell metabolism,cell signal transduction,immune system and genetic information analyzed by GO annotation and KEGG signal pathway enrichment analysis. mja-miR-34 can regulate the expression of target gene translation initiation factor in shrimp in vivo. These results indicated that mja-miR-34 and its target genes were involved in multiple cellular processes such as virus infection,but further verification was required. This study can provide data support and theoretical guidance for the characterization of mja-miR-34 target genes and the investigation of their biological functions.

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