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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2022年03期

Page:

35-45

Research Field:

化学

Publishing date:

Info

Title:

Polyphenols Response and Composition of Walnut Fruit at Different Parts Under Different Solvents Immersion

Author(s):

Wang Jihui¹; 2; Geng Yangyang¹; 2; Liu Yana¹; 2; Zhang Shixin¹; 2; Hu Bokai¹; 2; Liang Mei¹; 2; Tan Huamei¹; 2; He Jiali¹; 2

(1.Guizhou Institute of Walnut,Guiyang 550005,China)(2.Guizhou Academy of Forestry,Guiyang 550005,China)

Keywords:

ultrasound effect; solvent; walnut fruit; polyphenols; composition

PACS:

TS255.1

DOI:

10.3969/j.issn.1001-4616.2022.03.006

Abstract:

Studying the change polyphenols content of walnut diaphragma juglandis fructus and walnut shell to provide technical support for the utilization of walnut by-products. Analyzing the change and composition of polyphenols different parts of walnut fruit based on ultrasound effect by different solvent extraction. Secondly,the composition of polyphenols was identified by HPLC,and then the microstructure was observed by SEM. Walnut diaphragma juglandis fructus and shell polyphenols extraction conditions were optimized by Multivariate Quadratic Regression Equation Model. The better extraction conditions were as follows:solid to liquid ratio 1:59(g·mL^-1),treatment time 31 min,methanol concentration 67%,treatment temperature 61 ℃,ultrasound power 250 W. At this time,under methanol extraction the polyphenol yield of diaphragma juglandis fructus was(72.7±1.57)mg·g^-1. The regression equation was established:Y=71.12-0.079X₁+0.36X₂-0.13X₃+0.40X₄-0.91X₁X₂-0.17X₁X₃-0.31X₁X₄-0.45X₂X₃+0.23X₂X₄-0.60X₃X₄-2.10X²₁-1.42X²₂-0.68X²₃-1.35X²₄; solid to liquid ratio 1:85(g·mL^-1),treatment time 29 min,treatment temperature 61 ℃,ultrasound power 250 W. At this time,under water extraction polyphenol yield of diaphragma juglandis fructus was(58.06±1.62)mg·g^-1. The regression equation was established:Y=53.96+2.19X₁-2.31X₂+1.25X₄-1.08X₁X₂+0.66X₁X₄-0.62X₂X₄-2.68X²₁-5.76X²₂-2.91X²₄. Solid to liquid ratio 1:60(g·mL^-1),treatment time 31 min,methanol concentration 67%,treatment temperature 61 ℃,ultrasound power 300 W. At this time,under methanol extraction polyphenol yield of walnut shell was(20.04±1.32)mg·g^-1. The regression equation was established:Y=19.58+0.047X₁+0.35X₂-0.096X₃+0.55X₄-0.42X₁X₂-0.39X₁X₃+0.038X₁X₄-0.52X₂X₃+0.27X₂X₄-0.35X₃X₄-1.74X²₁-1.28X²₂-0.59X²₃-1.21X²₄; solid to liquid ratio 1:90(g·mL^-1),treatment time 37min,treatment temperature 55 ℃,ultrasound power 250 W. At this time,under water extraction polyphenol yield of walnut shell was(15.43±1.18)mg·g^-1. The regression equation was established:Y=13.77+0.45X₁-2.18X₂+0.36X₄-1.08X₁X₂-0.42X₁X₄+0.13X₂X₄-0.29X²₁-3.20X²₂+0.22X²₄. Through the HPLC analysis,under methanol and water extraction,two common kinds of monophenol identified in diaphragma juglandis fructus were gallic acid and catechin,gallic acid content was higher in water extraction and catechin content was higher in methanol extraction. Two common kinds of monophenol identified in the walnut shell were gallic acid and vanillic acid,gallic acid content was higher than methanol extraction,while the vanillic acid content is not much different between methanol and water extraction. The polyphenol yield was significantly higher in diaphragma juglandis fructus than walnut shell,the extraction effect of methanol solution is significantly better than that of pure water extraction. Scanning electron microscope of the surface of diaphragma juglandis fructus and walnut shell proved that ultrasound effect can significantly damage the surface tissue structures of diaphragma juglandis fructus and walnut shell,to promote the polyphenols to release more and faster,and dissolve into the extraction solution.

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Last Update: 2022-09-15