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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2023年02期

Page:

140-148

Research Field:

环境科学与工程

Publishing date:

Info

Title:

Effect of Soil Cd on Insect-Resistant Transgenic Rice Planting

Author(s):

Zhong Yanxin¹; Liu Yan²; Li Lei³; Qin Hongyi¹; Han Cheng³; Liu Biao⁴

(1.Zhongbei College,Nanjing Normal University,Danyang 212332,China)
(2.Suzhou Baixin Environmental Testing Engineering Technology Company Limited,Suzhou 215101,China)
(3.School of Geography,Nanjing Normal University,Nanjing 210023,China)
(4.Nanjing Institute of Environmental Science,Ministry of Ecology and Environment,Nanjing 210042,China)

Keywords:

insect-resistant transgenic rice;  cadmium stress;  oxidase activity;  Cd accumulation

PACS:

X173

DOI:

10.3969/j.issn.1001-4616.2023.02.018

Abstract:

The modification of insect-resistant gene is conducive to reduce insect pests and decrease pesticide and fertilizer application. Thus, the research on environmental adaptability of insect-resistant transgenic crops has important ecological significance. However, the adaptation potential of insect-resistant transgenic crops to heavy metal stress remains unclear. In this study, insect-resistant transgenic rice Huahui 1(HH1)and its non-transgenic parent rice Minghui 63(MH63)are selected for a pot experiment, and the soil Cd(II)concentrations are set as 0, 3.5, 60 and 240 mg·kg^-1, respectively. The agronomic traits, Cd content in plant organs, enzyme activity of rice roots and leafs, as well as physical and chemical properties of rhizosphere soil, are determined at tillering stage and maturity stage. The results show that compared with Cd₀, the plant height of HH1 and MH63 rice under Cd₂₄₀ treatment decrease by 35% and 46%, respectively. Their aboveground biomass decrease by 59% and 73% respectively, and their yield is reduced by 55% and 85%, respectively. Cd stress can significantly reduce plant height, biomass and yield of rice. Under the same Cd stress, the plant height, biomass and yield of HH1 rice are higher than those of MH63 rice, which indicate that the modification of exogenous Bt gene could improve the resistance of rice to Cd stress. The modification of exogenous Bt gene don't change the accumulation pattern of Cd in rice, however, under the same Cd stress, the Cd content in each organs of HH1 rice is higher than that of MH63 rice. The results of malondialdehyde(MDA)content showe that HH1 rice have better Cd resistance than MH63 rice at tillering stage, and the opposite was at maturity stage. The reason might be related to Cd concentration in plant organs, root microenvironment and detoxification enzyme system.In conclusion, Cd stress and exogenous gene modification affecte rice agronomic traits and Cd enrichment in plant organs. Moreover, HH1 rice at tillering stage have stronger Cd resistance than MH63 rice.

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Last Update: 2023-06-15