|Table of Contents|

Screening and Identification of the RTN4-C Interactive Proteins(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年01期
Page:
74-77
Research Field:
生命科学
Publishing date:

Info

Title:
Screening and Identification of the RTN4-C Interactive Proteins
Author(s):
Tang Xiaojun; Chen Yicun; Zhang Xiran
School of Life Science,Nanjing Normal University,The Key Laboratory of Molecular and Medical Biotechnology of Jiangsu Province,Nanjing 210097,China
Keywords:
RTN4- C;  RTN3;  yeast two-hyb rid;  immuno fluo rescence;  co- loco liza tion
PACS:
Q51-33
DOI:
-
Abstract:
Our prev ious study show ed tha t overexpression o f the RTN4- C m igh t inhib it SMMC7721 ce ll g row th and prom ote ce ll apoptosis. To investiga te the m echanism o f RTN4- C inh ib ition o f ce ll grow th and pro-apoptosis, the yeast two-hyb rid m ethod was adopted. By screen ing the mouse 7 - day Embryo MATCHMAKER cDNA Library, we obta ined the in terac ted prote in RTN3, wh ich has been reported to pro-apoptosis. Our further study ind ica ted that RTN4 - C and RTN3 co localized in HEK293 and H ela cells us ing immuno fluo rescence and fluo rescence vec to rs. The present study could be usefu l to c la rify the m echanism of RTN4- C inh ib ition o f ce ll grow th and pro-apoptosis

References:

[ 1] Fourn ierA E, G randPreT, Str ittm atte r SM. Identification of a receptorm ed ia ting Nogo- 66 inh ib ition o f axona l regeneration [ J]. Na ture, 2001, 409: 341-346.
[ 2] ChenM S, H uberA B, van derH aarMEW, e t al . Nogo- A is am ye lin-assoc iated neur ite outgrow th inh ib ito r and an antigen for monoclona l antibody IN- 1[ J]. Na ture, 2000, 403: 434-439.
[ 3] A cevedo L, Yu J, E rd jum ent-B romageH, et a.l A new ro le for Nogo as a regulator o f vascu lar remodeling[ J]. N atM ed,2004, 10( 4): 382-388.
[ 4] Li Q, Q i B, Oka K, et a.l L ink of a new type o f apoptosis- inducing gene ASY /Nogo- B to hum an cancer[ J]. Oncogene,2001, 20( 30) : 3 929-3 936.
[ 5] Oe rtle T, M erkler D, Schw abM E. Do cancer ce lls die because of Nogo- B? [ J] . Oncogene, 2003, 22( 9): 1 390-1 399.
[ 6] Chen Y C, Lu D D, Zhang X R, et a.l RTN4 - C g ene expression in hepatoce llular carc inom a and its influence on SMMC7721 cell g row th and apoptos is[ J]. Ac taG enn tica Sin ica, 2005, 32( 9): 891-897.
[ 7] Tagam i S, Eguch iY, K ino shitaM, et a .l A nove l prote in, RTN- xS, in terac ts w ith both Bc l- xL and Bc l- 2 on endop lasmic re ticulum and reduces the ir ant-i apoptotic activ ity[ J]. Oncog ene, 2000, 19: 5 736-5 746.
[ 8] Fourn ie rA E, G randPreT, S tritm ater SM. Identification o f a receptorm ediating Nogo- 66 inhib ition o f axona l regeneration[ J]. Na ture, 2001, 403: 439-444.
[ 9] H uW H, H ausm ann O N, YanM S, et a .l Identification and characteriza tion o f a nove lNogo- in teracting m itochondr ia l prote in ( N IMP) [ J]. J N euro chem, 2002, 81: 36-45.
[ 10] Taketom iM, K inosh ita N, K im ura K, et a.l Nogo- A expression in m ature o ligodendroctes o f  ra t spina l cord in association w ith spec ificm o lecules[ J]. Neurosc i Lett, 2002, 322: 37-40
[ 11] N ie D, Zhou Z, Ang B T, et a.l Nogo- A a tCNS paranodes is a ligand o f Caspr: possible regulation o fK+ channel location[ J]. EMBO J, 2003, 22: 5 666-5 678.
[ 12] L iu Q Z, GanM, Q i B, e t a.l D im eriza tion of tw o novel apoptosis- inducing pro teins and its function in regulating cell apoptosis[J] . Science in Ch ina ( Se ries C ), 2003, 46( 3): 225-235
[ 13] Qu X L, Q i Y P, Lan Q, et a .l The nove l endoplasm ic re ticulum ( ER)- targ eted pro tein HAP induces cell apoptosis by the depletion o f the ER Ca 2+ stores[ J] . FEBS Letters, 2002, 529: 325-331
[ 14] Kuang E, W an Q, Q i Y P, et a.l ER Ca 2+ depletion triggers apoptosis signa ls fo r endoplasm ic re ticulum ( ER) overload response induced by overexpressed reticu lon 3 ( RTN3 /HAP) [ J]. J Ce ll Phy sio ,l 2005, 204: 549-559
[ 15] Xu C Y, M a itre B B, Reed J C. Endoplasm ic reticulum stress: ce ll life and death dec isions[ J]. J C lin Invest, 2005,115( 10): 2 656-2 664.

Memo

Memo:
-
Last Update: 2013-05-05