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Expression of Recombinant Trichoderma Reesei Endoglucanase II in Escherichia coli and Properties of the Recombinant Enzyme(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年03期
Page:
92-98
Research Field:
生命科学
Publishing date:

Info

Title:
Expression of Recombinant Trichoderma Reesei Endoglucanase II in Escherichia coli and Properties of the Recombinant Enzyme
Author(s):
Luo JunGong XiaoyanWei WeiYang ChunFang HejianDai YijunYuan Sheng
Jiangsu Engineering and Technology Research Center for Industralization of Microbial Resources,School of Life Science,Nanjing Normal University,Nanjing 210046,China
Keywords:
CM C-Na endog lucanase E scher ichia coli βg lucan Trichod erm a reesei
PACS:
Q78
DOI:
-
Abstract:
Ce l5A ofT richoderma reesei w as fused w ith h istid ine( 6) tag and cloned in pET- 28a( + ) p lasm id. W ith a low tem pera ture induction stra tegy, the enzym ew as successfu lly expressed in E. co li host. W este rn b lot analysis show ed tha t the recomb inan tC el5A had am o lecu larw e ight of 43 000. The recom binant Ce l5A had m ax ima l ac tiv ities at pH 4. 5, 60℃ and w as stab lew hen it was heated fo r 1.5 h a t 65℃ . The enzym atic ac tiv ityw as enhanced by 1mmo l/LM n 2+ and inh ibited by Fe 3+ and Cu 2+ wh ile o ther m eta l ions and EDTA had no effec t. The recom binant Ce l5A was ab le to hydro-lyze βg lucan and CM C- Na, but unab le to hydro ly zeAv ice,l G lassm ic ro fiber filte rs, Xy lan, A rabiroxy lan and Xy log lu-can. The ac tiv ity of Cel5A to digest βg lucan is 6. 7-fo ld h igher than to d igest CMC-Na.

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Last Update: 2013-04-23