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Effective Simultaneous Separation and Purification of L-theanine and Poly-γ-glutamic Acid From Enzyme-Catalyzed Synthesis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2011年02期
Page:
90-93
Research Field:
生命科学
Publishing date:

Info

Title:
Effective Simultaneous Separation and Purification of L-theanine and Poly-γ-glutamic Acid From Enzyme-Catalyzed Synthesis
Author(s):
Xin Yinqiang; Lu Changyan; Wang Qi; Pu Hefang; Yin Zhimin
School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
Keywords:
L??thean ine;  PGA;  ????GGT;  ion exchange
PACS:
TQ922
DOI:
-
Abstract:
In enzym atic reaction w hich w as cata ly zed by GGT, g lutam ine and e thy lam ine we re used as substrates. The resu ltan t consists o f considerab le L thean ine and PGA, m ix ed w ith m ultip le impurities inc lude ch lo rid ion, ethy lam ine, g lu tam ine and g lu tam ate. Through ca tion an ion tandem exchange chrom a tog raphyw ith respective types of resin, L- thean  ine w as effec tive ly separated for further pur ification, accompan ied w ith acquisition of va luable byproduct PGA. Th is study prov ides the ev idence fo r larg e sca le produc tion o fL -theanine w ith recom binant  GGT.

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Memo

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Last Update: 2011-06-15