|Table of Contents|

Effect of Promoters on Expression of H5HA Avian Influenza Virus Gene in Potato Plant(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2012年01期
Page:
75-79
Research Field:
生命科学
Publishing date:

Info

Title:
Effect of Promoters on Expression of H5HA Avian Influenza Virus Gene in Potato Plant
Author(s):
Huang Cuixiang12Ji Qin2Zhang Yunfeng2Xu Chun2Dou Bingde2
1.Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
Keywords:
H5HA genetransgenic potatopromoter
PACS:
Q943.2
DOI:
-
Abstract:
The H5HA-encoding cDNA fragment of the avian influenza virus subtype H5N1 gene was,respectively,fused to the promoter fragments of potato granule-bound starch synthase I ( GBSSI) and sweet potato Sporamin A ( SpoA) . The recombinant genes were transfered into a potato cultivar( cv. Desiree) by using Agrobacterium-mediadate transformation. The transgenic plants were obtained and analyzed by PCR,Western blotting and Western dot blotting. The results were compared with the expression of the CaMV35S-H5HA recombinant gene in potato. The results showed that three recombinant proteins can be accumulated in potato tubers. The highest level of expression was the H5HA fused to GBSS I promoter, and the lowest one was the gene fused with the SpoA pomoter.

References:

[1] Chen H,Smith G J,Zhang S Y, et al. Avian Flu: H5N1 virus outbreak in migratory waterfowl[J]. Nature,2005, 436( 7048) : 191-192.
[2] 姜三杰,吴燕民. 转禽流感血凝素基因百脉根植株的获得及筛选[D]. 北京: 中国农业科学院,2010: 87.
[3] 韦小琼. 人禽流感研究进展及其应用策略[J]. 右江民族医学院学报,2006,28( 5) : 860-862.
[4] 颜渊清. 蛋白结构模拟预测H5 型禽流感病毒保守中和表位及其抗病毒多肽的初步设计[D]. 厦门: 厦门大学生命科学学院,2007: 88.
[5] 齐岩,孙凌霜,王彬. H5 亚型禽流感病毒HA1 基因在Sf9 昆虫细胞中的表达及其抗原性检测[J]. 中国兽医科学, 2006, 36( 5) : 352-356.
[6] 谭甜,季勤,窦秉德,等. 禽流感病毒H5HA 基因在马铃薯中的表达[J]. 南京师大学报: 自然科学版,2009,32( 3) : 104-108.
[7] Ji Q,Vincken J P,Suurs C J M, et al. Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis[J]. Plant Mol Biol,2003,51( 5) : 789-801.
[8] 萨姆布鲁克J,拉塞尔著D W. 分子克隆实验指南[M]. 北京: 科学出版社,2002: 238.
[9] 陈晶瑜,郭宝峰,何付丽,等. 适合双向电泳的植物全蛋白提取方法比较[J]. 中国农学通报,2010, 26( 23) : 97-100.
[10] 张晓薇. SDS-PAGE 对不同产地及质量枸杞的鉴别[J]. 研究光明中医, 2011,26( 5) : 917-918.

Memo

Memo:
-
Last Update: 2013-03-11