|Table of Contents|

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using Phthalic Acid Matrix for Small Molecule Analysis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
57-60
Research Field:
化学
Publishing date:

Info

Title:
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using Phthalic Acid Matrix for Small Molecule Analysis
Author(s):
Liu Hailong1; Huang Heyong2
(1.School of Life Science,Nanjing Normal University,Nanjing 210023,China) (2.Analysis and Testing Center,Nanjing Normal University,Nanjing 210023,China)
Keywords:
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-TOF MS); dopamine; matrix; Phthalic acid
PACS:
O657.63
DOI:
-
Abstract:
Phthalic acid was studied as matrices for determination of dopamine(153.08 Da)samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI TOF-TOF MS)method.This matrix material displayed at least two advantages.First,it was possible to avoid low mass chemical noise arising from the matrix in positive-ion mode.Second,phthalic acid could be used conventionally due to the low cost.The MALDI-TOF-MS analysis of dopamine demonstrated that the matrix material based on phthalic acid yielded spectra with minimal chemical noise and good analysis signal intensity.The present method can be readily applied to obtain the low background noise MALDI-TOF mass spectra of small molecules compounds.

References:

[1] Michael K,Doris B,Franz H.Influence of the wavelength in high irradiance ultraviolet laser desorption mass spectrometry of organic molecules[J].Anal Chem,1985,57(14):2 935-2 939.
[2]Tanaka K,Waki H,Ido Y,et al.Protein and polymer analyses up to m/z 100 000 by laser ionization time-of-flight mass spectrometry[J].Rapid Commun Mass Spectrom,1988,2(8):151-153.
[3]Karas M,Bahr U,Ingendoh A,et al.Principles and applications of matrix-assisted UV laser desorption/ionization mass spectrometry[J].Anal Chim Acta,1990,241(2):175-185.
[4]McCombie G,Knochenmuss R.Small-molecule MALDI using the matrix suppression effect to reduce or eliminate matrix background interferences[J].Anal Chem,2004,76(17):4 990-4 997.
[5]Tammy L,Jens O,Dietrich A V.An experimental comparison of electrospray ion-trap and matrix-assisted laser desorption/ionization post-source decay mass spectra for the characterization of small drug molecules[J].Rapid Commun Mass Spectrom,2001,15(8):608-614.
[6]Zhou X S,John J T,Claudia A,et al.Porous silicon as a versatile platform for laser desorption/ionization mass spectrometry[J].Anal Chem,2001,73(3):612-619.
[7]Chang S L,Ji H L,Kyoung K K,et al.Laser desorption/ionization-mass spectrometry using mesoporous silicate as matrix for the analysis of various molecules[J].Biotechnol Bioprocesses Eng,2007,12(2):174-179.
[8]Chang S L,Kyoung K K,June H K,et al.Analysis of small molecules by desorption/ionization on mesoporous silicate(DIOM)-mass spectrometry(MS)[J].Microporous Mesoporous Mater,2007,98(1):200-207.
[9]Kienast T,Heinz A.Dopamine and the diseased brain,CNS neurol[J].Disord:Drug Targets,2006,5(1):109-131.
[10]Ressler K,Nemeroff C B.Role of serotonergic noradrenergic systems in the pathophysiology of depression and anxiety disorders,depress[J].Anxiety,2000,12(1):2-19
[11]Conor P M,Sreenivasa R A,Waldemar G,et al.Modified silica-containing matrices towards the MALDI-TOF-MS detection of small molecules[J].International Journal of Mass Spectrometry Journal,2011,308(2/3):311-315.
[12]Michael D,Andy J T,Hari N.Controlling matrix suppression for matrix-assisted laser desorption/ionization analysis of small molecules[J].Rapid Commun Mass Spectrom,2004,18(17):1 885-1 888.

Memo

Memo:
-
Last Update: 2013-09-30