|Table of Contents|

Establishment of High Frequency Regeneration System of Phytolacca Americana(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
74-80
Research Field:
生命科学
Publishing date:

Info

Title:
Establishment of High Frequency Regeneration System of Phytolacca Americana
Author(s):
Wu Haojie1Yang Jie1Xu Yali1Yang Ziyi1Zheng Tiesong2Lu Changmei1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.Ginling College,Nanjing Normal University,Nanjing 210097,China)
Keywords:
Phytolacca americana Linn.regeneration systemexplantsadventitious budsrootingmedium
PACS:
Q945.5
DOI:
-
Abstract:
Phytolacca americana Linn.,which belongs to Phytolaccaceae,is a kind of perennial herb with high application value in industry,agriculture and medicine.In order to establish a stable and efficient regeneration system for P.americana,efficiency of callus induction,adventitious bud differentiation and elongation,and adventitious root induction etc.were analyzed from aspects of explants selection,hormones combination and applications of GA3 and AgNO3 etc..Results show that,the optimal explants for callus induction and bud differentiation are stem sections; the method of transplanting shoots in induction medium to sound seedling can significantly advance adventitious bud differentiation and promote bud proliferation coefficient.The optimal medium for adventitious bud differentiation is MS+6-BA 3.0 mg/L+NAA 0.01 mg/L+TDZ 0.02 mg/L with a multiplication coefficient up to 12.7.The optimal medium for adventitious buds elongation is MS+TDZ 0.02 mg/L+6-BA 3.0 mg/L+NAA 0.01 mg/L+GA3 2.0 mg/L.The optimal rooting medium is 1/2MS+NAA 0.4 mg/L with a rooting rate up to 91.7%.AgNO3 treatment inhibited the adventitious bud differentiation of P.americana.

References:

[1] 陈国菊,石丽,雷建军,等.中国商陆抗病毒蛋白基因的克隆及其转化辣椒[J].园艺学报,2008(6):847-852.
[2]李景原,王太霞,杨相甫,等.商陆单细胞平板培养及色素高产细胞株的筛选[J].天然产物研究与开发,2000(4):62-65.
[3]施和平,梁朋,权宏.商陆毛状根的诱导、培养及其皂甙的产生[J].生物工程学报,2003(1):46-49.
[4]薛生国,陈英旭,骆永明,等.商陆(Phytolacca acinosa Roxb.)的锰耐性和超积累[J].土壤学报,2004(6):889-895.
[5]徐向华,施积炎,陈新才,等.锰在商陆叶片的细胞分布及化学形态分析[J].农业环境科学学报,2008(2):515-520.
[6]刘庆,刘慧君.商陆的应用及毒副作用[J].新疆中医药,2002(1):40-42.
[7]舒世坤,舒迎澜.美洲商陆的组织培养[J].植物生理学通讯,1987(2):50-51.
[8]崔丽华,张海燕,张铁汉,等.美洲商陆快速繁殖实验体系的建立[J].北京师范大学学报:自然科学版,2004(3):390-392.
[9]邹利娟,苏智先,胡进耀,等.美洲商陆组培快速繁殖[J].中药材,2008(9):1 299-1 301.
[10]徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂[J].植物学通报,2003(2):227-237.
[11]康培婧,李梅兰,李洪清.TDZ诱导的蓝猪耳根高效再生体系(简报)[J].亚热带植物科学,2005(2):64.
[12]Metz T D,Dixit R,Earle E D.Agrobacterium tumefaciens mediated transformation of broccoli(Brassica oleracea var.italica)and cabbage(Boleracea var.capitata)[J].Plant Cell Reports,1995,15:287-292.
[13]Kuvshinov V,Koivu K,Pehu E.Biotechnology and Genetic Resources Applied in Oil-seed and Vegetable Brassica Improvement[M]//Watanabe K N,Pehu E.Plant biotechnology and plant genetic resources for sustainability and productivity.USA:Academic Press,1997:197-206.
[14]Palmer C E.Enhanced shoot regeneration from Brassica campestris by silver nitrate[J].Plant Cell Reports,1992,11:541-549.
[15]韦健琳,秦新民.大白菜的组织培养研究[J].广西师范大学学报:自然科学版,2000(1):85-87.
[16]沈海龙.植物组织培养[M].北京:中国林业出版社,2005:42.
[17]李浚明.植物组织培养教程[M].北京:中国农业大学出版社,1992:79.

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Last Update: 2013-09-30