|Table of Contents|

Cloning and Characterization of Heat Shock Protein 90 Gene Introns in the Chinese Mitten Crab Eriocheir sinensis

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年04期
Page:
107-
Research Field:
生命科学
Publishing date:

Info

Title:
Cloning and Characterization of Heat Shock Protein 90 Gene Introns in the Chinese Mitten Crab Eriocheir sinensis
Author(s):
Zhang RufengSong JinyuanLi PengLu ZhouYan JieWu YeGao Qing
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
Eriocheir sinensisheat shock protein 90(HSP90)intronsequence analysis
PACS:
Q95
DOI:
-
Abstract:
The heat shock protein 90(HSP90)is a highly conserved molecular chaperone particularly important in the maturation of a diverse set of proteins that regulating key steps in various biological processes,such as hormone signaling pathways in cells,cell cycle regulation,cell growth and development,etc..Introns are the noncoding DNA fragments,which are involved in variety of biological activities,such as regulating gene expression,protein alternative splicing and gene transcription,etc..Based on PCR technique,the three introns(296 bp,141 bp and 173 bp)of a HSP90 gene(named as EsHSP90)were identified and characterized from the Chinese mitten crab Eriocheir sinensis.EsHSP90 gene had two GT-AG type introns(intron-1 and intron-3).While exon-intron splicing for intron-2 did not follow the GT-AG or AT-AC rules.The full-length DNA of EsHSP90 is 3,126 bp in size and contains an open reading frame of 2,157 bp which encodes a 718 amino acid polypeptide(82.85 kDa)with HSP90 family signatures and ATP binding domain.Sequence alignment shows that the full-length DNA sequence of EsHSP90 shared more than 80% identity with HSP90 sequences reported in crustaceans.

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Last Update: 2013-12-30