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eIF4E Promotes Drug Resistance in Non-small Cell Lung Carcinoma(PDF)


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eIF4E Promotes Drug Resistance in Non-small Cell Lung Carcinoma
Li MinChen XiMa ZhuoWu DandanNi PingZhai SulanLi PingZou MeijuanWang Xuerong
Department of Pharmacology,School of Medicine,Nanjing Medical University,Nanjing 210029,China
NSCLCeIF4Eerlotinibdrug resistanceEGFR
R965. 2;R734. 2
To observe the effects of eIF4E on the proliferation of erlotinib resistant cells and mechanism. Paired erlotinib sensitive and resistant HCC827-EP and HCC827-ER cells were used. Protein and mRNA expression levels were detected by Western blot and quantitative RT-PCR. The 5 ds growth curve and inhibition effects of erlotinib were determined by SRB assay. Downregulation of eIF4E expression were achieved by transfection of cells with siRNA targeting eIF4E using lipofectamine,and nonsense siRNA were used as control. eIF4E expression was increased in HCC827-ER cells compared with HCC827-EP cells. And downregulation of eIF4E expression inhibited the growth of HCC827-ER cells(P<0. 05)and sensitized their reponses erlotinib possibly through downregualtion of eIF4E expression( P <0. 05). Downregulation of eIF4E expression inhibited the growth of NSCLCs and enhanced the inhibitory effects of erlotinib.


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Last Update: 2014-06-30