|Table of Contents|

Establishment and Characteristics of Baby Skin Explant Fibroblast(PDF)

《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

Issue:
2014年03期
Page:
100-
Research Field:
生命科学
Publishing date:

Info

Title:
Establishment and Characteristics of Baby Skin Explant Fibroblast
Author(s):
Wang Jiamin1Sheng Wuling2Ping Ling2Xu Shuilin1Zhao Bin2Ma Zhongren1Qiao Zilin1
(1.Gansu Engineering Research Center for Animal Cell,Lanzhou 730030,China)(2.College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030,China)
Keywords:
baby skinfibroblast cell linecharacteristics
PACS:
Q813.1+1
DOI:
-
Abstract:
The baby skin explants were cultured with tissue pieces method.The primary cells were purified and preserved with liquid nitrogen after being passaged to the thrid generation.Observations on morphology,dynamic growth,microbial contamination,analysis of karyotype,isoenzyme of lactate dehydrogenase and fluorescin plasmid transfection and expression were carried out.The results showed that the cell line was fibroblast and growed well.The maximum proliferation concentration was 1.64×105/mL and poulation doubling time of cells(PDT)was 63.12 h.The tests for bacteria,fungi,virus and mycoplasma were all negative.The chromosomes were 46 and diploid cells were dominant of 86%.The banding patterns of the isoenzyme of lactate dehydrogenase had significant difference and there were five bands in all.The exogenous plasmid could copy and express in the cell.Those showed that the cell line was established successfully,which lays the foundation of the work bases for composite artificial skin tissue engineering and provides the experimental conditions for skin diseases.So this has the important theoretical and practical significance.

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Last Update: 2014-09-30