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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2014年04期

Page:

89-

Research Field:

生命科学

Publishing date:

Info

Title:

Knockdown of FLT-1,KDR by Adenovirus-Delivered SmallInterfering RNA Inhibits Angiogenesis

Author(s):

Xu Jianfeng¹; Sheng Weigan²; Peng Wanxin²

(1.Jiangsu Province Blood Center,Nanjing 210042,China)(2.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)

Keywords:

angiogenesis; FLT-1; KDR; RNAi

PACS:

Q291

DOI:

-

Abstract:

This study used the homologous recombination technique constructed a recombinant adenovirus AdH1-siRNA/FLT-1 and AdH1-siRNA/KDR targeting FLT-1 and KDR infects human endothelial cells(HUVEC),observation the effect of the adenovirus interfere the proliferation of HUVEC in vitro and the tube formation.Reverse transcription-PCR showed that this adenovirus could specifically decrease level of the FLT-1 and KDR mRNA of HUVEC.Compared with control group,the FLT-1 and KDR mRNA expression of decreased to 40% and 52%.AdH1-siRNA/FLT-1 and KDR can efficiently interfere the proliferation of HUVEC,9 d after infection of adenovirus,the average cell number of control group and vector group are 31.5×10⁴/μL and 28×10⁴/μL,that of groups respectively infected with AdH1-siRNA/VEGF are 21.5×10⁴/μL,and can efficiently interfere the tube formation of HUVEC on matrigel.Averagely,the tube number of every HPF is 10.75 in the control group,and 10.25 in the vector group.In the group treated with AdH1-siRNA/VEGF,there are 7 tubes in the HPE.It is sowed that AdH1-siRNA/FLT-1 and AdH1-siRNA/KDR can also interfere tube formation.

References:

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Last Update: 2014-12-31