|Table of Contents|

EffectofRNAInterferenceTargetingEgr-1InhibiteTetrandrine-InducedAutophagyandApoptosisofPancreaticPaTu8988Cellsinvitro(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2015年03期
Page:
71-
Research Field:
生命科学
Publishing date:

Info

Title:
EffectofRNAInterferenceTargetingEgr-1InhibiteTetrandrine-InducedAutophagyandApoptosisofPancreaticPaTu8988Cellsinvitro
Author(s):
PengWanxin1WanYanya2GeLu1JinJie1GongAihua1WuChaoyang2
(1.DepartmentofBiology,SchoolofMedicine,JiangsuUniversity,Zhenjiang212013,China)
(2.DepartmentofRadiationOncology,AffiliatedPeople’sHospital,JiangsuUniversity,Zhenjiang212013,China)
Keywords:
pancreaticcancertetrandrineEgr-1autophagycellapoptosis
PACS:
R730.5
DOI:
-
Abstract:
HumanpancreaticcancercellsPaTu8988weretransfectedwithsiRNA-ScrambleandsiRNA-Egr-1for24hrespectively,thentreatedwithtetrandrineforanother24h.Treatedbydifferentconcentrationoftetrandrine,thecytotoxiceffectoftetrandrinewasexaminedbyCCK-8,andexpressionofautophagy-relatedproteinweredetectedbyWesternblot.Theexpressionofcellautophagy-andapoptosis-relatedproteinweredetectedbyWesternblot.Alloftheresultsindicatedthatcomparingwithcontrolgroup,siEgr-1significantlyattenuatestetrandrine-inducedautophagyandpromotestheapoptosisofPaTu8988cells.

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Last Update: 2015-09-30