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Study on Anti-Tumor Activity and Mechanisms of Blf-ADT(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2016年01期
Page:
67-
Research Field:
生命科学
Publishing date:

Info

Title:
Study on Anti-Tumor Activity and Mechanisms of Blf-ADT
Author(s):
Wang QianPan WangLi WeiXiao ZhiyiPan LiliXu Guanglin
School of Life Sciences,Nanjing Normal University,Jiangsu Province Key Laboratory for Molecularand Medical Biotechnology,Nanjing 210023,China
Keywords:
HeLaBlf-ADTcell proliferationcell apoptosisanti-tumor effect
PACS:
Q4
DOI:
-
Abstract:
The purpose of this study was to observe the activity and mechanisms of a new compound Blf-ADT on HeLa cells. The effects of Blf-ADT on Hela cells was investigated by morphological observation,MTT assay and colony formation assay,respectively. Wound healing assay was used to detect the effect of Blf-ADT on the migration of HeLa cells. Meanwhile,the activities of Blf-ADT on apoptosis was measured by Flow cytometry. Furthermore,Western blot was performed to investigate the expressions of related proteins in HeLa cell line treated with Blf-ADT. As a result,we found that Blf-ADT could inhibit cell proliferation and reduce cell migration in a dose-and time-dependent manner. In addition,Blf-ADT could significantly increase the rate of apoptosis and affect the expression of apoptosis-related proteins. Taken together,the data indicate that Blf-ADT can significantly inhibit the growth of HeLa cells and have potential clinical value for treatment of cervical cancer.

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Last Update: 2016-03-30