|Table of Contents|

PGC-1β Antagonizes LPS-Induced Hepcidin Expressionand Iron Accumulation in Liver(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2016年03期
Page:
67-
Research Field:
·生命科学·
Publishing date:

Info

Title:
PGC-1β Antagonizes LPS-Induced Hepcidin Expressionand Iron Accumulation in Liver
Author(s):
Zhang Wenxiang; Xu Qi; Chen Siyu; Liu Chang
School of Life Sciences，Nanjing Normal University，Nanjing 210023，China
Keywords:
PGC-1β; Hepcidin; iron metabolism; inflammation; liver
PACS:
Q291
DOI:
10.3969/j.issn.1001-4616.2016.03.012
Abstract:
This study is aimed to investigate the effect of PGC-1β on LPS-induced Hepcidin expression and iron accumulation in liver. RT-qPCR and Western Blot analyses were used to detect the expression levels of PGC-1β and Hepcidin in mouse liver and primary hepatocytes treated with LPS. Gain- and loss-of-function studies were performed to assess the role of PGC-1β in the regulation of Hepcidin expression under basal and LPS-stimulated conditions. Finally，Perls’ Prussian blue staining was administrated to detect the effects of PGC-1β on LPS-induced hepatic iron deposition. Results showed that，（1）LPS caused a significant reduction in PGC-1β expression，whereas increased Hepcidin expression both in vivo and in vitro；（2）Over-expression of PGC-1β inhibited LPS-induced Hepcidin expression and intracellular iron accumulation in liver. PGC-1β antagonizes LPS-induced Hepcidin expression and iron accumulation in liver.

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Last Update: 2016-09-30