|Table of Contents|

FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年01期
Page:
76-
Research Field:
·生命科学·
Publishing date:

Info

Title:
FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum
Author(s):
Li Haimei1Wang Yingying1Gao Tao2Xu Cunfa3Shi Zhiqi2Chen Jian2Xu Xiaofeng1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.Institute of Food Safety and Nutrition,Jiangsu Academy of Agriculture Science,Nanjing 210014,China)(3. Central Laboratory,Jiangsu Academy of Agriculture Science,Nanjing 210014,
Keywords:
Fusarium graminearumPDKosmotic stressglycerolcell death
PACS:
S432.1
DOI:
10.3969/j.issn.1001-4616.2018.01.014
Abstract:
Fungi’s HOG(high osmolarity glycerol)pathway in response to osmotic stress has been revealed. However,the upstream signaling regulator of the pathway remains unclear. In this study,we investigated the pivotal role of a mitochondrial energy metabolic gene encoding for pyruvate dehydrogenase kinase(FgPDK1)in Fusarium graminearum in the regulation of NaCl stress by using genetic and physiological-biochemical approaches. The main results have been obtained as follows,(1)Determination of hyphal growth indicated that ΔFgPDK1(an FgPDK1 knock-out mutant)was more sensitive than wild type(PH-1).(2)Compared to PH-1,NaCl stress induced more electrolyte leakage and higher accumulation of glycerol in the hyphae of ΔFgPDK1.(3)ROS accumulation,MDA content,and cell death increased significantly in ΔFgPDK1 as compared to PH-1 under NaCl stress.(4)The relative expression levels of anti-oxidative genes(SOD and CAT)were significantly lower in ΔFgPDK1 as compared to PH-1 under NaCl stress.(5)All of the above physiological and biochemical responses could be recovered to the level of PH-1 in ΔFgPDK1-C(a gain-of-function complementary strain of ΔFgPDK1). These results suggested that FgPDK1 played important roles in the regulation of HOG downstream pathway and the maintenance of anti-oxidative capacity in F.graminearum under high osmatic stress. This study provides new evidence for understanding the molecular mechanism in fungi’s osmatic stress adaption.

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Last Update: 2018-03-31