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Analysis of Ran and DDT Resistance in Drosophila Melanogaster Kc Cells(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年02期
Page:
83-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Analysis of Ran and DDT Resistance in Drosophila Melanogaster Kc Cells
Author(s):
Bo Qian; Chen Lu; Chang Chunjie; Liu Yahui; Cheng Luogen
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
Ran; DDT; stress; resistance; apoptosis
PACS:
Q965.9
DOI:
10.3969/j.issn.1001-4616.2018.02.015
Abstract:
In this paper,the Kc cells of Drosophila melanogaster were stimulated by Dichlorodiphenyltrichloroethane(DDT),we focus on the changes in the expression of Ran. Real- time fluorescence quantitative PCR was used to detect the changes in gene expression of Ran in Kc cells under different concentrations of DDT induced stimulation. Flow cytometry was used to detect the apoptosis rate of cells with different concentrations of DDT. The results showed that the expression of Ran in the cells treated by 20 mg/L DDT was the highest.At the same time,the apoptosis rate decreased obviously at this concentration,the trend of cell apoptosis was basically consistent with the change of Ran expression.DDT can significantly increase the expression of Ran gene in Kc cells and reduce the rate of apoptosis. In order to further verify the function of Ran,we carried out the overexpression and interference of Ran.The cell apoptosis was detected.The results showed that the interference of Ran could significantly increase the rate of apoptosis. The experiment showed that Ran had a certain protective effect on the cells.Ran may be one of the genes associated with insecticide tolerance,which provides a basis for further analysis of the stress response and molecular toxicology of insect pests.

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Last Update: 2018-11-06