|Table of Contents|

Changes of Muscle-Specific microRNAs in Circulation andDifferent Tissues in the Mouse Overtraining Model(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2019年04期
Page:
103-110
Research Field:
·生命科学·
Publishing date:

Info

Title:
Changes of Muscle-Specific microRNAs in Circulation andDifferent Tissues in the Mouse Overtraining Model
Author(s):
Xu Lin1; 2; Wang Jinzhi1; 2; Zheng Yili4; Tian Dong2; Zhang Haipeng1; Xu Shengjia2; Yao Zengqiang3; Ma Jizheng2
(1.Department of Exercise and Heath,Nanjing Sport Institute,Nanjing 210014,China)(2.The Research Center of Military Exercise Science,The Army Engineering University of PLA,Nanjing 211101,China)(3.School of Physical Education,Nanjing Medical University,N
Keywords:
microRNAs; serum; liver; muscle; overtraining
PACS:
G804.7
DOI:
10.3969/j.issn.1001-4616.2019.04.015
Abstract:
The purpose of this study was to explore whether muscle-specific microRNAs(miRNAs)can be used as a potential biomarker to monitor overtraining and to provide reference significance for preventing overtraining. Forty-five C57BL/6J male mice,6 weeks old,were randomly divided into control group(CON),exercise group(NT)and overtraining group(OT),with 15 rats in each group. After training according to different 8-week exercise plans,the overtraining model of mice was successfully constructed. The mice were serum,gastrocnemius,myocardium and liver after 24 hours of rest. Real-time quantitative PCR(RT-qPCR)was used to detect the changes of seven muscle-specific miRNAs in different tissues.(1)After 8 weeks of training,the NT incremental load test scores increased significantly(P<0.05),but there was no significant difference in OT(P>0.05),and the OT score was significantly lower than NT(P<0.05).(2)Compared with CON,serum miR-133a,-133b,and -208a decreased in both exercise groups,but decreased significantly in OT(P<0.05). There was no significant difference between the two exercise groups(P>0.05).(3)Compared with CON,the levels of miR-1,-133a in the gastrocnemius muscles decreased in the two exercise groups,among which NT decreased significantly(P<0.05),OT had no significant difference(P>0.05),and the two exercise groups there was no significant difference(P>0.05).(4)There were no significant differences in the seven muscle-specific miRNAs in the liver of the three experimental groups(P>0.05).(5)Compared with CON,the level of miR-499 in the two exercise groups was significantly lower(P<0.05),but there was no significant change between the two exercise groups(P>0.05); miR-133 in the NT heart,there was a significant increase in 133b(P<0.05),but there was no significant difference between the two exercise groups(P>0.05). Among them,the level of miR-133a in the heart was significantly increased in OT compared with CON(P<0.05). To sum up,this experiment found that the levels of miR-1,miR-133a,miR-133b and miR-208a in different tissues have different degrees of significant changes,myomiRs can be used as a potential marker for monitoring overtraining,reflecting its physiological process.

References:

[1] 王金之,张震,徐盛嘉,等. 运动监控在过度训练综合征中的研究现状[J]. 体育科技文献通报,2017,25(6):62-64.
[2]ALLEN D L,BANDSTRA ER,HARRISON B C,et al. Effects of spaceflight on murine skeletal muscle gene expression[J]. J Appl Physiol,2009(106):582-595.
[3]PEREIRA B C,PAULI J R,De SOUZA C T,et al. Eccentric exercise leads to performance decrease and insulin signaling impairment[J]. Med Sci Sports Exerc,2014,46(4):686-94.
[4]马继政,孙飙. 运动和MicroRNAs[J]. 体育科研,2013,34(6):65-68.
[5]NIELSEN S,SCHEELE C,YFANTI C,et al. Muscle specific microRNAs are regulated by endurance exercise in human skeletal muscle[J]. J Physiol,2010(588):4029-37.
[6]MA Z,QI J,MENG S,et al. Swimming exercise training-induced left ventricular hypertrophy involves microRNAs and synergistic regulation of the PI3K/AKT/mTOR signaling pathway[J]. Eur J Appl Physiol,2013(113):2473-86.
[7]BARTEL D P. MicroRNAs:genomics,biogenesis,mechanism,and function[J]. Cell,2004(116):281-297.
[8]RAMASAMY S,VELMURUGAN G,SHANMUGHA R K,et al. MiRNAs with apoptosis regulating potential are differentially expressed in chronic exercise-induced physiologically hypertrophied hearts[J]. PLoS One,2015,10:e0121401.
[9]MOOREN F C,VIERECK J,KRüGER K,et al. Circulating micrornas as potential biomarkers of aerobic exercise capacity[J]. Am J Physiol Heart Circ Physiol,2014,306(4):H557.
[10]NAVICKAS R,GAL D,LAUCEVIACˇGIUS A,et al. Identifying circulating microRNAs as biomarkers of cardiovascular disease:a systematic review[J]. Cardiovasc Res,2016,111(4):322-337.
[11]LIU J,LIANG X,ZHOU D,et al. Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit[J]. EMBO Mol Med,2016(8):1212-1228.
[12]MIRZAEI H R,SAHEBKAR A,MOHAMMADI M,et al. Circulating microRNAs in hepatocellular carcinoma:potential diagnostic and prognostic biomarkers[J]. Curr Pharm Des,2016,22(34):5257-69.
[13]da ROCHA A L,PEREIRA B C,PAULI J R,et al. Downhill running-based overtraining protocol improves hepatic insulin signaling pathway without concomitant decrease of inflammatory proteins[J]. PloS One,2015,10(10):e0140020.
[14]WOJTASZEWSKI J F,RICHTER E A. Effects of acute exercise and training on insulin action and sensitivity:focus on molecular mechanisms in muscle[J]. Essays Biochem,2006,42:31-46.

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Last Update: 2019-12-31