|Table of Contents|

The Effect of Down-Regulation of TMEM16A Expressionon Skeletal Muscle Cell Differentiation(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2020年03期
Page:
106-111
Research Field:
·生物学·
Publishing date:

Info

Title:
The Effect of Down-Regulation of TMEM16A Expressionon Skeletal Muscle Cell Differentiation
Author(s):
Yuan Wen; Fan Chun’e; Chen Yuchen; Xu Yanhua; Zhang Qianqian; Sun Zhiyuan; Chen Huaqun
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
TMEM16A; T16A inh-A01; C2C12; myoblast; myogenin
PACS:
Q291
DOI:
10.3969/j.issn.1001-4616.2020.03.017
Abstract:
TMEM16A(Transmembrane Protein 16A)is one of the calcium(Ca2+)activated chloride channels(CaCCs). It is widely expressed in a variety of tissues and has important physiological functions. So far,the role of TMEM16A in the development of skeletal muscle has not been determined. In this study,we reported for the first time the effects down-regulation of TMEM16A on myogenic differentiation of myoblasts by using of the specific inhibitor T16Ainh-A01. We found that TMEM16A was apparently expressed both in myoblast cell line C2C12 and murine primary myoblast. We then determined that T16Ainh-A01 apparently inhibited the myogenic differentiation of both C2C12 and primary myoblasts. Furthermore,we showed that the expression of myogenin was obviously reduced in T16Ainh-A01 treated cells. Our results suggest that the calcium activated chloride channel activity of TMEM16A is involved in the myogenic differentiation of myoblast,the mechanisms under is still need to be investigated.

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Last Update: 2020-09-15