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¡¶ÄϾ©Ê¦´óѧ±¨£¨×ÔÈ»¿Æѧ°æ£©¡·[ISSN:1001-4616/CN:32-1239/N]

Issue:

2022Äê03ÆÚ

Page:

68-78

Research Field:

ÉúÎïѧ

Publishing date:

Info

Title:

Analysis of Selection Pressure on TLR1 and TLR9 Genes in Perciformes

Author(s):

Liu Xing¹; Gao Bo²; Ji Hongjiu²; Jia Chaofeng²; Zhu Fei²; Meng Qian²;  Chen Shuyin²; Zhang Zhi yong²; Xu Shixia¹

(1.School of Life Sciences,Nanjing Normal University,Jiangsu Provincial Key Laboratory of Biodiversity,Nanjing 210023,China)(2.Marine Fisheries Research Institute of Jiangsu Province,Jiangsu Key Laboratory for Genetics and Breeding of Marine Fishes,Nantong 226007,China)

Keywords:

Perciformes; TLR1; TLR9; adaptive evolution; innate immunity

PACS:

Q953

DOI:

10.3969/j.issn.1001-4616.2022.03.010

Abstract:

The Perciformes is an order with the highest species richness in fish,distributed in many waters,and plays an essential role in developing the fishery economy. However,the high-density culture mode aggravates the fish diseases caused by pathogenic microorganisms. Toll-like receptors(TLRs)are essential proteins in innate immunity and play an important role in the defense against bacterial or viral infections. In this paper,TLR1 and LTR9 were used as candidate genes,and the homologous sequences of 17 representative species of Perciformes were downloaded. The molecular evolutionary basis of innate immunity of this species was discussed by evolutionary analysis. Selective pressure analysis showed that the three methods simultaneously detected 16 and 8 positive selection sites in TLR1 and TLR9,respectively. 86% of sites were located in the Leucine rich repeat(LRR)domain. These results suggest that Perciformes species face the challenge of high pathogenic microbial infection. These positive selection sites may lead to critical functional changes in gene evolution to resist pathogen invasion. In addition,free-ratio and branch-site models found that positive selection of genes was species-specific. For example,TLR1 is significantly positively selected in the last common ancestor(LCA)branch of Sparidae and Percidae,and TLR9 is also significantly positively selected in the last common ancestor branch of Sparidae. These lineages are often reported to be threatened by pathogens. Therefore,the positive selection of multiple Perciformes lineages may result from evolution in long-term resistance to pathogenic microorganisms. The above results indicated that TLR1 and TLR9 of Perciformes species may have changed their adaptive functions during the long-term resistance to pathogenic microorganisms.

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Last Update: 2022-09-15