|Table of Contents|

Bioinformatics Analysis of Enterocytozoon hepatopenaei Septin and Its Potential Role in Proliferation(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2025年01期
Page:
66-74
Research Field:
生态学
Publishing date:

Info

Title:
Bioinformatics Analysis of Enterocytozoon hepatopenaei Septin and Its Potential Role in Proliferation
Author(s):
Zhang Sheng1; Qiao Yi2; Cao Xiaohui2; Zhang Leiting3; Cheng Jie2; Wu Hailong1; Shen Hui2
(1.School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222000,China)
(2.Jiangsu Marine Fisheries Research Institute,Nantong 226007,China)
(3.School of Marine and Engineering,Nanjing Normal University,Nanjing 210023,China)
Keywords:
Enterocytozoon hepatopenaei; Septin; prokaryotic expression; chromogenic in situ hybridization
PACS:
S945.4
DOI:
10.3969/j.issn.1001-4616.2025.01.009
Abstract:
Enterocytozoon hepatopenaei(EHP)is one of the significant pathogens in the shrimp aquaculture industry,but the molecular mechanisms of its division and proliferation remain unclear. This study aimed to elucidate the potential role of the EhpSeptin protein during EHP division by cloning and analyzing the gene sequence,expression,and localization of EhpSeptin during the division period of EHP. The results indicated that the EhpSeptin gene sequence encoded a protein with 265 amino acids,a molecular weight of approximately 30.4 kDa,and an isoelectric point of 4.75. An EhpSeptin prokaryotic expression vector was constructed,and high-purity expressed protein was obtained. Western blotting confirmed the consistency of the expressed protein with the native protein. Additionally,this study established chromogenic in situ hybridization(CISH)for the EhpSeptin gene to analyze its expression pattern in infected shrimp samples. The results demonstrated that the expression level of EhpSeptin increased with the intensity of EHP infection,suggesting its involvement in the EHP division process. This study is the first to report the cloning,expression,localization,and variations in the EhpSeptin protein during EHP infection,providing a theoretical foundation and technical means for exploring the molecular mechanisms of EHP division and preventing infection by this pathogen.

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Last Update: 2025-02-15