|Table of Contents|

Molecular Mechanism of CD11b Regulated IL-12Expression in LPS-Stimulated Macrophages(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年02期
Page:
71-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Molecular Mechanism of CD11b Regulated IL-12Expression in LPS-Stimulated Macrophages
Author(s):
Liu Shan; Song Zhengwen; Zheng Chenchen; Zhang Guoxiu; Zhao Zhihui
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
CD11b; interleukin 12; lipopolysaccharides; macrophage; JNK; NF-κB
PACS:
R363.2
DOI:
10.3969/j.issn.1001-4616.2018.02.013
Abstract:
CD11b is a member of the leukocyte integrin family,which plays a significant role in the pathogenesis of inflammation. In an endotoxemia mouse model,we found that lipopolysaccharide(LPS)caused a significant decrease of interleukin-12(IL-12)in peripheral blood,and pretreatment with CD11b inhibitor effectively prevented the occurrence of such event. To elucidate the underlying molecular mechanism that CD11b regulates the production of IL-12 under LPS stimulation,we performed further studies using RAW264.7 cells. Results from Enzyme-linked immunosorbent assay,real-time quantitative PCR,and Western Blot demonstrated that,CD11b inhibited the expression of IL-12 in LPS-stimulated RAW264.7 cells. Knocking down the expression of CD11b increased the expression of IL-12p35 and IL-12p40 at both transcription and protein levels in RAW264.7 cells stimulated with LPS. The regulation of CD11b on IL-12 expression was closely associated with the activation of JNK and NF-κB signaling pathways,while p38 and ERK signaling pathways were less involved. Thus,this study suggested that CD11b inhibited IL-12 production in LPS-stimulated macrophages mainly via JNK and NF-κB signaling pathways,and inhibiting the function of CD11b may be of help for attenuating excessive inflammatory responses induced by LPS.

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Last Update: 2018-11-06