|Table of Contents|

Progress in Gene Editing Methods—Taking the E.coli Gene Knockout Method as an Example(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年03期
Page:
102-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Progress in Gene Editing Methods—Taking the E.coli Gene Knockout Method as an Example
Author(s):
Xue FanWei HuixianHu JiaweiWang Jinjun
College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China
Keywords:
Escherichia coligene modificationred homologous recombinationCRISPR/Cas technology
PACS:
Q348
DOI:
10.3969/j.issn.1001-4616.2018.03.016
Abstract:
Escherichia coli is an important research object of molecular biology,it is the main microbe who can produce amino acids,organic acids and recombinant proteins and other substances. In molecular biology experiments,we do not need a complete sequence of Escherichia coli gene,then how to intercept the gene sequence become worthy of study. Red homologous recombination is the traditional method of gene knockout of Escherichia coli. The RecA and RecBCD proteins are mainly encoded by RecA homologous recombination system,which mediates DNA for homologous recombination. After several technological innovation,there are still many deficiencies. Genomics editing includes the three major technologies,TALEN,ZFN and CRISPR/Cas. One of the CRISPR/Cas technology is the world’s most promising revolutionary genetic modification technology.

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Last Update: 2018-11-19