|Table of Contents|

Localization and Function of Cyc2 in Schizosaccharomyces pombe(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2020年02期
Page:
108-113
Research Field:
·生物学·
Publishing date:

Info

Title:
Localization and Function of Cyc2 in Schizosaccharomyces pombe
Author(s):
Yang Guihong; Huang Ying
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
Saccharomyces pombe; mitochondrial; Cyc2; location
PACS:
R331.3+1
DOI:
10.3969/j.issn.1001-4616.2020.02.017
Abstract:
The CYC2 protein in budding yeast is related to the assembly of Cyt c,which is of great significance to the function of mitochondria. But in Schizosaccharomyces pombe,the function of the protein Cyc2 which is the homologous protein of CYC2 has not been known. In this paper,the location and function of Cyc2 were studied. The homologous recombination method was used to construct Δcyc2 mutants,it was observed the phenotype of Δcyc2 strain on non-fermentation medium(glycerol plate),and construct a complementing strain to verify the phenotype. As a result,Δcyc2 showed growth defects on the glycerol plate. By detecting the expression of respiratory streptavidin encoded by the mitochondrial genome,it was found that the deletion of cyc2 does not affect the expression of respiratory streptavidin. The sequence of Cyc2 was analyzed by bioinformatics and the result showed that the N-terminus of Cyc2 contained a mitochondrial localization sequence(MTS)and the protein has no transmembrane region. In order to determine the specific localization of the protein in the cell,it was constructed Cyc-GFP and Tom20-Flag/Cyc2-Myc strains,respectively,and identified the protein as a mitochondrial inner membrane peripheral protein by fluorescence observation and biochemical methods. To sum up,Cyc2 is a mitochondrial inner membrane peripheral protein,which does not affect the expression of mitochondrial respiratory chain protein,and may affect the function of mitochondria through other channels.

References:

[1] KWON Y Y,CHOI K M,CHO C,et al. Mitochondrial efficiency-dependent viability of saccharomyces cerevisiae mutants carrying individual electron transport chain component deletions[J]. Molecules and cells,2015,38(12):1054-1063.
[2]MALINA C,LARSSON C,NIELSEN J. Yeast mitochondria:an overview of mitochondrial biology and the potential of mitochondrial systems biology[J]. FEMS yeast research,2018,18(5):40.
[3]BAKER B M,HAYNES C M. Mitochondrial protein quality control during biogenesis and aging[J]. Trends in biochemical sciences,2011,36(5):254-261.
[4]BABU M,VLASBLOM J,PU S,et al. Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae[J]. Nature,2012,489(7417):585-589.
[5]ALLEN J W. Cytochrome c biogenesis in mitochondria—Systems III and V[J]. The FEBS journal,2011,278(22):4198-4216.
[6]CORVEST V,MURREY D A,BERNARD D G,et al. C-type cytochrome assembly in Saccharomyces cerevisiae:a key residue for apocytochrome c1/lyase interaction[J]. Genetics,2010,186(2):561-571.
[7]BERNARD D G,QUEVILLON C,MERCHANT S,et al. Cyc2p,a membrane-bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes[J]. The journal of biological chemistry,2005,280(48):39852-39859.
[8]CORVEST V,MURREY D,HIRASAWA M,et al. The flavoprotein Cyc2p,a mitochondrial cytochrome c assembly factor,is a NAD(P)H-dependent haem reductase[J]. Molecular microbiology,2012,83(5):968-980.
[9]PEARCE D A,CARDILLO T S,SHERMAN F. Cyc2p is required for maintaining ionic stability and efficient cytochrome c import and mitochondrial function in Saccharomyces cerevisiae[J]. FEBS letters,1998,439(21128):307-311.
[10]DUMONT M,SCHLICHTER J,CARDILLO T,et al. CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c[J]. Molecular and cellular biology,1993,13(10):6442-6451.
[11]MCALLISTER S,POLSON S,BUTTERFIELD D,et al. Validating the Cyc2 neutrophilic iron oxidation pathway using meta-omics of zetaproteobacteria iron mats at marine hydrothermal vents[J]. mSystems,2020,5(1):553-559.
[12]SANCHEZ N S,PEARCE D A,CARDILLO T S,et al. Requirements of Cyc2p and the porin,Por1p,for ionic stability and mitochondrial integrity in Saccharomyces cerevisiae[J]. Archives of biochemistry and biophysics,2001,392(2):326-332.
[13]MAYR J A,HAACK T B,FREISINGER P,et al. Spectrum of combined respiratory chain defects[J]. Journal of inherited metabolic disease,2015,38(4):629-640.
[14]SOUBANNIER V,VAILLIER J,PAUMARD P,et al. In the absence of the first membrane-spanning segment of subunit 4(b),the yeast ATP synthase is functional but does not dimerize or oligomerize[J]. The journal of biological chemistry,2002,277(12):10739-10745.
[15]SOUBANNIER V,RUSCONI F,VAILLIER J. The second stalk of the yeast atp synthase complex:identification of subunits showing cross-links with known positions of subunit 4(Subunit b)[J]. Biochemistry,1991,38(45):15017-15024.
[16]ZHANG C,WANG R,LIU Z,et al. The plant triterpenoid celastrol blocks PINK1-dependent mitophagy by disrupting PINK1’s association with the mitochondrial protein TOM20[J]. The journal of biological chemistry,2019,294(18):7472-7487.

Memo

Memo:
-
Last Update: 2020-05-15