|Table of Contents|

Study on Tissue Culture and Rapid Propagation TechnologySystem of Polygonatum sibiricum Red(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2020年03期
Page:
129-135
Research Field:
·生物学·
Publishing date:

Info

Title:
Study on Tissue Culture and Rapid Propagation TechnologySystem of Polygonatum sibiricum Red
Author(s):
Tian Huai1; Hou Na2
(1.Guizhou Forestry School,Guiyang 550201,China)(2.Guizhou Academy of Forestry,Guiyang 550201,China)
Keywords:
Polygonatum sibiricum; tissue culture; rapid propagation; seed
PACS:
Q942
DOI:
10.3969/j.issn.1001-4616.2020.03.020
Abstract:
In order to establish a high-efficiency tissue culture propagation and rooting system for wild polygonatum sibiricum,and provide a feasible key technical solution for its factory and large-scale nursery,this article uses sterile wild polygonatum sibiricum seeds as test materials to study the effects of seed germination,subculture,multiplication,rooting and transplanting of refined seeds. The results showed that the best medium for seed germination and start of polygonatum sibiricum was MS+6-BA(0.2 mg/L)+2,4-D(0.2 mg/L)+GA3(2.0 mg/L),the seed germination rate was 20%,and the buds were about 90 ds later. It is 0.50 mm long and grows well. The best medium for subculture of callus is MS+6-BA(4.0 mg/L)+2,4-D(0.2 mg/L)+TDZ(0.2 mg/L),callus the growth is the best,the bud spots are multiplying,and the seedlings are strong; the best medium for multiplying culture is MS+6-BA(2.0 mg/L)+TDZ(0.2 mg/L)+NAA(0.2 mg/L),the buds grow well and elongate large,green leaves,strong seedlings. The best medium for rooting induction culture is 1/2 MS+TDZ(0.01 mg/L)+NAA(0.2 mg/L),the rooting rate is up to 70%,and the root number and root length are the best,respectively,14 pieces,0.60 cm. The survival rate of transplanting 4-8 roots after transplanting seedlings is up to 92%. The experimental results believe that using sterile seeds of polygonatum sibiricum as a cultivation object can obtain a large number of tissue culture seedlings of polygonatum sibiricum in a short time,which is beneficial to solve the problem of shortage of seedlings in planting and production.

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Last Update: 2020-09-15