|Table of Contents|

Isolation, Identification and uation of an Antagonistic Bacteria of VPAHPND Strain(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2023年01期
Page:
73-79,87
Research Field:
生物学
Publishing date:

Info

Title:
Isolation, Identification and uation of an Antagonistic Bacteria of VPAHPND Strain
Author(s):
Liu Xueshi1; Jiang Ge2; Shen Hui2; Qiao Yi2; Chen Jie2; Cao Xiaohui2; Dou Yabin3; Wen Mengying3; Wan Xihe2; Wang Libao2; Li Hui2; Shen Wenjun2
(1.College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China)
(2.Jiangsu Institute of Oceanology & Marine Fisheries, Nantong 226007, China)
(3.College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China)
Keywords:
Vibrio parahaemolyticus;  Litopenaeus vannamei;  antagonistic bacterium;  Pseudomonas aeruginosa
PACS:
Q939.9
DOI:
10.3969/j.issn.1001-4616.2023.01.011
Abstract:
Acute hepatopancreatic necrosis disease causes serious economic losses to the Litopenaeus Vannamei shrimp industry in recent years. Studies have shown that the pathogen responsible for this disease is a type of Vibrio containing PirAB virulence factors,specifically Vibrio parahaemolyticus(VPAHPND). In this paper,the antagonistic strain of VPAHPND was isolated from Nibea albiflora in the coastal waters of Jiangsu province,with identified by morphological characteristics,physiological and biochemical characteristics,and 16S rDNA sequence analysis. Its antagonistic effect and antagonistic active substances were preliminarily studied,and the assessment of bio-safety were preliminarily optimized. The results showed that the strain JSHY97 with powerful antagonistic effect was screened and identified as Pseudomonas aeruginosa. JSHY97 had an antagonistic effect against 3 strains of VPAHPND with a zone of inhibition diameter of 19.25 mm-21.80 mm,and its active substances were preliminarily judged to be extracellular products. After 7 days of diet,protection rates of 80% were also achieved by feeding JSHY97 to L. vannamei. The copy number of the virulence gene PirA in the JSHY97-fed L.vannamei was significantly lower than that in the control group(P<0.05). Safety experiments showed that JSHY97 was non-toxic to Brachydanio reriovar and L. vannamei. Pseudomonas aeruginosa strain JSHY97 has a good inhibitory effect on VPAHPND,which provides a new strain resource for the prevention and treatment of AHPND.

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Last Update: 2023-03-15