[1]霍爱玲,张晨岭,李崇奇,等.中国野猪核DNA SANDX-2位点遗传变异分析[J].南京师大学报(自然科学版),2007,30(03):79-86.
　Huo Ailing,Zhang Chenling,Li Congqi,et al.Genetic Variation of Anonymous Nuclear DNA SANDX-2 in Chinese Wild Boars(Sus scorfa)[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(03):79-86.
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中国野猪核DNA SANDX-2位点遗传变异分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第30卷
期数:
2007年03期
页码:
79-86
栏目:
生命科学
出版日期:
2007-09-30

文章信息/Info

Title:
Genetic Variation of Anonymous Nuclear DNA SANDX-2 in Chinese Wild Boars(Sus scorfa)
作者:
霍爱玲1;  张晨岭1;  李崇奇2;  陈建琴3;  周开亚1;  常  青1
( 1. 南京师范大学遗传资源研究所, 江苏南京210097 )
( 2. 海南医学院基础医学部, 海南海口571101)
( 3. 江苏教育学院生物系, 江苏南京210016)
Author(s):
Huo Ailing1; Zhang Chenling1; Li Congqi2; Chen Jianqin3; Zhou Kaiya 1; Chang Qin1
1.Genetic Resource Institute,Nanjing Normal University,Nanjing 210097,China
2. Departm en t of BasicM ed ical Science, H ainanM edical College, H a ikou 571101, Ch ina
 3. Departm en t of B iology, J iangsu In stitute of Educat ion, Nan jing 210016, Ch ina
关键词:
中国野猪;  SANDX-2位点;  遗传变异
Keywords:
Ch inese w ild boa r;  SANDX - 2;  genetic va riation
分类号:
Q953
摘要:
研究测定了89个中国野猪和1个家猪未知功能核基因SANDX-2位点的序列.在序列比对后长510bp左右,发现有2处4种类型碱基缺失,30个位点发生碱基替换,共定义了19种单元型.在野猪中共发现18种单元型,其中7种只在某一地方种群中发现,11种为地方种群间共享单元型.用邻接法基于核苷酸Kimura 2-parameter模型构建系统发生树中,19种单元型被明显分为2大枝.尽管各枝得到的Bootstrap值均较低,且各地方种群中出现的单元型散布在不同的进化枝中,但单元型在野猪亚种内的分布已出现了一定的地理分布格局.若不考虑碱基的插入/缺失,用TCS构建19种单元型网络表明,HAP2单元型为一古老单元型,其他为衍生单元型.
Abstract:
The anonym ous nuclear DNA SANDX - 2 locus o f 89 indiv idua ls o f Ch inese w ild boars and a dom estic p ig were am plified and sequenced fo r study ing the genetic va riab ility. A tota l o f 510 bp w ere ob ta ined a fter a ligned, inc luding 4 inde ls and 30 substitutions. E ighteen hap lo types we re de fined in Ch inese w ild boars, am ongw hich 7 haplo types we re observed only in one loca l popu la tion, and 11 hap lotypesw ere sha red by tw o o rm ore loca l popu la tions. The phy log enetic tree constructed by the ne ighbor-jo in ingm ethod based on K imura 2- param eters model div ided the hap lo types into tw o distinct clades. A lthough the trees w ere no t suppo rted by high bootstrap va lue, and the haplotypes in different popu lation w ere lo ca ted in d if ferent clades, there are g eog raph ica l d istribution patterns in the Ch inese w ild boars. Ignor ing the gaps in the sequences, the nesting cladogram of 19 hap lo types suggested that HAP2 m ay be ancestra l haplotype

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备注/Memo

备注/Memo:
基金项目: 江苏省生物多样性与生物技术重点实验室基金资助项目.
作者简介: 霍爱玲( 1976- ) , 女, 硕士研究生, 主要从事动物分子生物学的学习与研究. E-m ail:huoailing- hn@ 163. com
通讯联系人: 常青( 1966- ) , 副教授, 博士, 主要从事动物分子系统学的教学与研究. E-m ail:ch angq@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05