[1]沈萍,刘维红,闫淑珍,等.XG32菌株产ACC脱氨酶的培养条件和酶活影响因素[J].南京师大学报(自然科学版),2008,31(01):104-108.
　Shen Ping,Liu Weihong,Yan Shuzhen,et al.Culture Conditions and Character of Extracellular Enzyme ACC Deaminase Excreted by Bacterium Strain XG32[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(01):104-108.
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XG32菌株产ACC脱氨酶的培养条件和酶活影响因素()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年01期
页码:
104-108
栏目:
生命科学
出版日期:
2008-03-30

文章信息/Info

Title:
Culture Conditions and Character of Extracellular Enzyme ACC Deaminase Excreted by Bacterium Strain XG32
作者:
沈萍;刘维红;闫淑珍;陈双林;
南京师范大学生命科学学院, 江苏南京210046
Author(s):
Shen Ping;  Liu Weihong;  Yan Shuzhen;  Chen Shuanglin
School of Life Science, Nanjing Normal University, Nanjing 210046, China
关键词:
细菌;  ACC脱氨酶;  产酶条件
Keywords:
bacter ium;  ACC deam inase;  enzym atic activ ity
分类号:
Q93
摘要:
为了明确具1-氨基环丙烷-1-羧酸(1-aminocyclopropane-1-carboxylate ACC)脱氨酶活性细菌菌株XG32的产酶条件和酶活影响因素,以ACC为底物,研究了诱导培养基中底物的浓度、温度、pH对该菌株产酶的影响及产酶的时程;分别在不同温度、不同pH及添加8种金属离子的条件下测定了酶的活力和稳定性.结果表明:菌株XG32在有底物ACC存在时才能被诱导产酶,温度上升至5℃及pH>5·5时酶活性才开始被诱导,在25℃和pH7·0~8·0时酶活性最高;诱导初始至24h内是酶产生的上升期, 24 h后酶产生呈下降趋势. 酶的最适反应温度为30℃ , 高于35℃ 酶的活力下降, 在65℃ 酶的活力趋于零; 该酶在pH 7.5~ 9. 5 之间有较好的稳定性; 金属Cu 2+ 、Zn 2+ 对ACC 脱氨酶有激活作用, 而H g 2+ 、Ag+ 则有抑制作用. 因此, 认为ACC 脱氨酶酶活性的出现对底物的存在非常敏感, 在自然界中少量的酶就可以催化ACC的分解. 但酶活力受条件影响较大, 仅能在一定的范围内保持酶活的稳定性.
Abstract:
In orde r to investigate optim al cu lture conditions and the charac ter o fACC( 1-am inocy clopropane- 1 carboxy-l ic ac id) deam inase excreted by bac terium stra in XG32, exper im ents were done to de term ine the e ffect on the enzym a tic ac tiv ity under different cond itions, inc luding concentration of inducerACC in cu lture m ed ium, culture tem pera ture and pH, w hile tim e process o f ACC deam inase w as a lso assayed. Then we m easured the activ ity and stability of the enzym e under d ifferent temperatures, pH and m eta ls. The results ind icated tha t the ACC deam inase could be induced on ly when the inducer ex isted and the tem peraturew as abov e 5℃ , and pH w as above 5.5. The enzym atic y ield reached the highest when the cu lture temperature w as 25℃ and cu lture pH was 7.0 to 8. 0. The enzym a tic y ie ld constantly increased in 24 hours after inducer was added, and then dec lined. A s for activ ity, the optim al temperatu re of ACC deam inase was 30℃approx im ately. The enzym atic activ ity a lm ost d isappeared w hen it was 65℃ . The enzym e had a preferab le stab ility a t pH 7. 5~ 9. 5. M oreov er, ions Cu 2+ and Zn 2+ cou ld activate the ACC deam inase, w hileH g 2+ and Ag+ cou ld inhib it the enzym e ac tiv ity. In conc lusion, theACC deam inase is sensitive to the substance. Thatm eans a little o f this kind o f enzym e can decom pose ACC in natural env ironm ent. How ever, the enzym e is sensitive to the circumstance, and its stab ility is on ly a t a certa in ex tens ion.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 江苏省基础研究计划( BK2007222 )资助项目.
作者简介: 沈萍( 1983-) , 女, 硕士研究生, 研究方向: 土壤微生物应用. E-m ail: spf ly@ 163. com
通讯联系人: 闫淑珍( 1963- ) , 女, 副教授, 研究方向: 微生物的应用. E-m ail:yanshu zhen@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05