[1]宋向阳,欧阳嘉,蔡梅松,等.限制性供氧对木糖发酵制备乙醇的影响[J].南京师大学报(自然科学版),2010,33(04):63-67.
　Song Xiangyang,Ou Yangjia,Cai Meisong,et al.Effect of Limited Providing Oxygen on Fermentation of Xylose to Ethanol[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(04):63-67.
点击复制

限制性供氧对木糖发酵制备乙醇的影响()
分享到：

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年04期
页码:
63-67
栏目:
化学
出版日期:
2010-12-20

文章信息/Info

Title:
Effect of Limited Providing Oxygen on Fermentation of Xylose to Ethanol
作者:
宋向阳;欧阳嘉;蔡梅松;勇强;
南京林业大学化学工程学院, 林木遗传与生物技术省部共建教育部重点实验室, 江苏南京210037
Author(s):
Song Xiangyang; Ou Yangjia; Cai Meisong; Yong Qiang
College of Chemical Engineering,Nanjing Forestry University,Key Laboratory of Forest Genetic & Biotechnology ofMinistry of Education,Nanjing 210037,China
关键词:
限制性供氧;  木糖;  发酵;  乙醇
Keywords:
lim ited prov iding oxygen;  xylose;  fermentation;  ethano l
分类号:
TQ223.122
摘要:
以树干毕赤酵母为发酵菌株,木糖为发酵底物,通过限制性供氧发酵木糖制备乙醇.结果表明,搅拌转速和通风量是影响传氧系数的重要变量.搅拌转速对传氧系数的影响大于通风量.在275~350 r/min内搅拌转速的改变可引起传氧系数的显著改变.在通风量为2.0 L/L?h,通过改变搅拌转速调节供氧量,当搅拌转速为350 r/min时发酵效果最佳,木糖利用率为91.7%,乙醇浓度达14.3 g/L.当通风量为3.0 L/L?h,搅拌转速为300 r/min时,木糖利用率和乙醇浓度均达到最大值,分别为96.3%和15.0g /L.
Abstract:
Ferm enta tion o f xy lose to ethano lw as investiga ted w ith P ichia stip itis by lim ited prov id ing oxygen. The results showed that the speed of stirring and a ir ra tew as m ain factor fo r oxygen transfer ra te. E ffect o f stirr ing speed on oxygen transfer rate w asm ore than tha t of a ir ra te. The chang e o f stirr ing speed led to g reat change o f a ir rate between 275 and 350 round per m inute. To contro l oxygen transfer rate by the change o f stirring speed when a ir ra tew as 2.0 L /L?h. It w as the best e ffect of stirring speed at 350 r /m in. The rate o f consum ed xy losew as 91. 7%. The concentration of ethano l reached 14.3 g /L. W hen a ir rate was 3. 0 L /L? h and stirring speed w as 300 r/m in, the rate of consum ed xy lose and stirring speed both reached max im um values which w ere 9613%, 15.0 g /L respec tive ly.

参考文献/References:

[ 1] Bru inenberg PM, de Bot P H M, van D ijken J P, e t a.l NADH- linked a ldo se reductase: The key to anaerob ic a lcoholic ferm enta tion o f xy lose by yeasts[ J]. ApplM icrobio l B iotechno,l 1984, 19( 25): 256-260.
[ 2] De lg enes J P, M o letta R, Nav arro JM. Ferm enta tion of D-xy lose, D-g luco se, L- arab ino se m ixture by Pich ia stipitis: e ffect o f the oxygen transfer rate on ferm entation pe rfo rm ance[ J]. B io techno l B ioeng, 1989, 34: 398-402.
[ 3] RizziM, H arwa rt K, Bu-i Thanh N A, et a.l A k ine tic study o f the NAD+ -xy lito-l dehydrogenase from the yeast P ichia stipitis [ J] . J Ferm en t B ioeng, 1989, 67: 25-30.
[ 4] R izz iM, K le in C, Schu lze C. Xy lose ferm entation by yeast. 5. Use o f ATP ba lances fo rm ode ling oxygen- lim ited grow th and ferm entation of yeast P ich ia stip itis w ith xy lo se as ca rbon source[ J] . B iotechnol Bioeng, 1989, 34: 509-514.
[ 5] 宋向阳, 陈牧, 毛连山, 等. 戊糖己糖混合糖发酵生产乙醇的主要影响因素[ J] . 南京师大学报: 自然科学版, 2009, 32 ( 1): 115-119.
[ 6] Je ffr ies T W, Ku rtzm an C P. Stra in se lection, taxonom y, and genetics of xylose- ferm enting yeasts[ J]. Enzym eM icrob Techno, l 1994, 16: 922-932.
[ 7] Sanchez S, et a .l Comparative study o f the ferm enta tion o f D-g lucose /D-x ylose m ixtures w ith Pachyso len tannoph ilus and Candida sheha tae[ J]. B ioprocess Eng ineer ing, 1999, 21: 525-532.
[ 8] Sanchez S, et a .l The ferm entation of m ix tures o f D-g lucose and D-xy lose by Candida sheha tae, Pich ia stip itis or Pachyso len tannoph ilus to produce e thano l[ J]. J Chem Techno l B iotechno,l 2002, 77: 641-648.
[ 9] M iller G L. Use o f din itrosa licy lic ac id reagent fo r determ ination of reducing sugar[ J]. Anal Chem, 1959, 31( 3): 426-428.
[ 10] 季更生, 勇强, 余世袁. 发酵抑制物对树干毕赤酵母戊糖发酵的影响[ J] . 南京林业大学学报: 自然科学版, 2007, 31 ( 6): 133-135.
[ 11] 高孔荣. 发酵设备[M ]. 北京: 轻工业出版社, 1991: 116-119.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(30871992);“863”计划资助(2008AA05Z401) 通讯联系人: 宋向阳, 博士, 副教授, 研究方向: 生物化工. E-mail:xiangyangsong@ hotmail. com
更新日期/Last Update: 2013-04-08