[1]纪元,侯北伟,罗玉明.苦瓜基因组DNA提取方法的比较研究[J].南京师大学报(自然科学版),2012,35(02):89-92.
　Ji Yuan,Hou Beiwei,Luo Yuming.Comparison of DNA Extraction Methods From Leaves of Momordica Charantia(Cucurbitaceae)[J].Journal of Nanjing Normal University(Natural Science Edition),2012,35(02):89-92.
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苦瓜基因组DNA提取方法的比较研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第35卷
期数:
2012年02期
页码:
89-92
栏目:
生命科学
出版日期:
2012-06-20

文章信息/Info

Title:
Comparison of DNA Extraction Methods From Leaves of Momordica Charantia(Cucurbitaceae)
作者:
纪元1; 2; 侯北伟1; 罗玉明1
( 1． 南京师范大学生命科学学院，江苏南京210046) ( 2． 淮阴师范学院生命科学学院，江苏省环洪泽湖生态农业生物技术重点实验室，江苏淮安223300)
Author(s):
Ji Yuan1; 2; Hou Beiwei1; Luo Yuming1; 2
1.School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
苦瓜; DNA 提取; 改良CTAB 法; SRAP
Keywords:
Momordica charantia Linn． ; DNA extraction; modified mehods of CTAB; SRAP
分类号:
S642.5
摘要:
针对苦瓜富含多酚类化合物和多糖等物质的特点,以新鲜苦瓜叶片为材料,分别采用改良CTAB法、QIAGEN试剂盒、CTAB法提取基因组DNA,并对DNA进行纯度和浓度的检测,同时开展了SRAP验证.结果表明:使用改良CTAB法从苦瓜叶片中提取基因组DNA,其纯度高、质量好、相对分子量大,多酚类化合物和多糖等杂质去除比较完全.SRAP标记验证显示,条带清晰、多态性好.因此改良CTAB法适用于高质量提取苦瓜基因组DNA,并能满足SRAP等分子标记对DNA的要求.
Abstract:
Because of polysaccharides and polyphenolic and other interfering compounds in leaves of Momordica charantia ( Cucurbitaceae) ，the genomic DNA extraction of methods namly modified CTAB，Dneasy Kit and CTAB were compared． Then the quality and purified of extracted DNA was detected，and high quality DNA was measured by sequencerelated amplified polymorphism ( SRAP) markers． The results showed that modified CTAB moethod could be used to extract DNA from leaves of M． charantia，with high qulity，high purify，high relative molecular mass and low interfering compounds． SPAP markers detection was also shown clear bands and good polymorphism． So，the modified CTAB method was suitable for the extraction of genomic DNA from M． charantia，which was also available to further SRAP or other molecular markers study．

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 江苏省环洪泽湖生态农业生物技术重点实验室开放课题( HZHL1007) ．通讯联系人: 罗玉明，教授，研究方向: 植物资源与生物技术． E-mail: yumingluo@163． com
更新日期/Last Update: 2013-03-11