[1]唐刘秀,许志强,葛家春,等.中华绒螯蟹3个育种基础群体遗传特征的微卫星分析[J].南京师大学报(自然科学版),2013,36(02):84-90.
　Tang Liuxiu,Xu Zhiqiang,Ge Jiachun,et al.Microsatellite Analysis on the Genetic Structure of Three Breeding Stocks of Eriocheir Sinensis[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(02):84-90.
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中华绒螯蟹3个育种基础群体遗传特征的微卫星分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年02期
页码:
84-90
栏目:
生命科学
出版日期:
2013-06-30

文章信息/Info

Title:
Microsatellite Analysis on the Genetic Structure of Three Breeding Stocks of Eriocheir Sinensis
文章编号:
1001-4616(2013)02-0084-07
作者:
唐刘秀1; 2; 许志强2; 葛家春1; 2; 3; 陈乙荣4; 赵沐子2; 杨家新1
1.南京师范大学生命科学学院,江苏 南京 210023
2.江苏省淡水水产研究所,江苏 南京 210017
3.南京淳一水产科技发展有限公司,江苏 高淳 211316
4.高淳县固城湖石臼湖管理委员会,江苏 高淳 211300
Author(s):
Tang Liuxiu1; 2; Xu Zhiqiang2; Ge Jiachun1; 2; 3; Chen Yirong4; Zhao Muzi2; Yang Jiaxin1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
2.Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China
3.Nanjing Purity Aquaculture Development Co.,Ltd.,Gaochun 211316,China
4.Gaochun Gucheng Shijiu Lakes Administration Committee,Gaochun 211300,China
关键词:
中华绒螯蟹; 微卫星; 育种基础群体; 遗传多样性
Keywords:
Eriocheir sinensis; microsatellite; base stocks for breeding; genetic diversity
分类号:
Q953
文献标志码:
A
摘要:
为进行长江水系中华绒螯蟹优良品系的选育,本研究利用16对微卫星引物对长江水系中华绒螯蟹3个育种基础群体(第一群体采自长江口崇明团结沙,第二群体采自长江扬中江段,第三群体采自长江靖江、六合、江浦江段)共计89个个体的遗传特征进行分析.结果表明:3个中华绒螯蟹基础群体均具有较高的遗传多样性(其总体平均期望杂合度He为0.701 4).各群体在16个微卫星位点上的观察杂合度(Ho)均高于期望杂合度(He),第一群体、第二群体和第三群体的平均观察杂合度分别为0.775 0、0.745 7、0.791 7,平均期望杂合度分别为0.705 0、0.702 9、0.696 4.第一群体的平均期望杂合度最高,第三群体最低,各群体间无显著性差异(P>0.05).3个基础群体在16个微卫星位点上的固定指数Fis多为负值,其中第三群体的Fis最小(-0.156 1),第二群体的Fis最大(-0.079 6),提示本研究中各群体内部存在着较为明显的远缘繁殖现象.3个群体的Hardy-Weinberg平衡偏离指数(D)在-0.379 4～0.966 6,每个群体均有5个微卫星位点存在杂合子缺失现象.本研究中各群体间遗传距离介于0.032 1～0.038 4,其中第一群体与第二群体间的遗传距离最小(0.032 1),第二群体与第三群体间的遗传距离最大(0.038 4).总体而言,本次研究所选的3个基础群体遗传多样性指数均较高,适合作为选育的材料.
Abstract:
In order to select excellent germplasm of Eriocheir sinensis,16 pairs of microsatellite primers were used to analyze the genetic characters of 3 base stocks which were collected from Chongming Tuanjiesha of Yangtze River Delta,Yangzhong section,and Jingjiang,Liuhe,Jiang Pu sections of Yangtze River respectively.The results showed that the genetic diversity indices of the 3 stocks are high with average expected heterozygosities(He)as 0.701 4,and the average observed heterozygosities(Ho)of the 3 stocks were higher than the average expected heterozygosities(He)in the all 16 microsatellite loci.The average observed heterozygosities(Ho)of the 3 stocks were 0.775 0,0.745 7 and 0.791 7,and the average expected heterozygosities(He)were 0.705 0,0.702 9,and 0.696 4 respectively.The first stock had the highest He,while the third stock had the lowest value,but there was no significant difference among the three stocks(P>0.05).The fixation indices(Fis)were used to evaluate the inbreeding or outcrossing of the 3 stocks,and the negative values were observed at almost all 16 microsatellite loci for the 3 stocks.The Hardy-Weinberg equilibrium(D)among the three stocks ranged from -0.379 4 to 0.966 6,and there were heterozygous reduction at five locus in each of these stocks.The pairwised genetic distance among the three stocks ranged from 0.032 1 to 0.038 4.The Ds between the first and second stock was smaller(0.032 1)than any others and it can be inferred that the genetic exchange between them was more frequent.Overall,the three base stocks in the present study had relatively higher genetic diversities,and it was suitable for selective breeding.

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备注/Memo

备注/Memo:
收稿日期:2012-12-19.
基金项目:江苏省农业科技自主创新资金项目(cx(10)125).
通讯联系人:葛家春,研究员,研究方向:水产动物育种.E-mail:gjc09@hotmail.com
更新日期/Last Update: 2013-06-30