[1]扶庆权,周 峰,华 春,等.微波辅助提取海蓬子总黄酮工艺的响应曲面法优化[J].南京师大学报(自然科学版),2013,36(02):96-103.
　Fu Qingquan,Zhou Feng,Hua Chun,et al.Microwave-assisted Extraction of Total Flavonoids from Salicornia Bigelovii Torr.Optimized by Response Surface Methodology[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(02):96-103.
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微波辅助提取海蓬子总黄酮工艺的响应曲面法优化()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年02期
页码:
96-103
栏目:
生命科学
出版日期:
2013-06-30

文章信息/Info

Title:
Microwave-assisted Extraction of Total Flavonoids from Salicornia Bigelovii Torr.Optimized by Response Surface Methodology
文章编号:
1001-4616(2013)02-0096-08
作者:
扶庆权1; 周 峰1; 华 春1; 王仁雷2
1.南京晓庄学院生物化工与环境工程学院,江苏 南京 211171
2.江苏教育学院生命科学与化学学院,江苏 南京 210013
Author(s):
Fu Qingquan1; Zhou Feng1; Hua Chun1; Wang Renlei2
1.School of Biochemical and Enviromental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China
2.School of Life Sciences and Chemistry School,Jiangsu Institute of Education,Nanjing 210013,China
关键词:
海蓬子; 总黄酮; 微波辅助提取; 响应曲面法
Keywords:
Salicornia bigelovii Torr.; total flavonoids; microwave-assisted extraction; response surface methodology
分类号:
TS201.1
文献标志码:
A
摘要:
本研究对海蓬子总黄酮的提取工艺条件进行了优化,通过单因素试验考察乙醇浓度、微波功率、提取时间、料液比和pH对总黄酮得率的影响.在单因素试验的基础上,采用响应曲面法优化微波辅助提取海蓬子总黄酮的最佳工艺条件.结果表明,微波辅助提取海蓬子总黄酮的最佳工艺条件是:乙醇浓度85%(V/V)、微波功率105 W、微波温度70 ℃、微波时间14 min、料液比1:55、pH 10.5,此时海蓬子总黄酮得率达到6.81%,与模型预测值6.89%基本相同.
Abstract:
The extraction conditions of total flavonoids from Salicornia bigelovii Torr.were optimized.Effects of ethanol concentration,microwave power,solid-liquid ratio,extraction time and pH value on the extraction of total flavonoids’were studied by single-factor experiments.Based on the single-factor test,the optimal extraction conditions of total flavonoids from Salicornia bigelovii Torr.were obtained through the response surface methodology.The results showed that the optimum extraction conditions of total flavonoids from the Salicornia bigelovii Torr.were adding 80 times amount 85% ethanol and extracting for 14 minutes at 70 ℃ and pH 10.5,the extraction rate of total flavonoids was 6.89% under the optimal extraction conditions.This result was basically consistent with the predictive value(i.e.6.81%)of the fitted equation.

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备注/Memo

备注/Memo:
收稿日期:2012-10-21.
基金项目:江苏省自然科学基金青年基金项目(BK2012073)、国家高技术研究发展863计划(2012AA021701).
通讯联系人:周峰,副教授,博士,研究方向:盐生植物资源开发与利用.E-mail:zfibcas@163.com
更新日期/Last Update: 2013-06-30