[1]张入峰,宋金远,李 鹏,等.中华绒螯蟹热激蛋白90基因内含子的克隆与界定[J].南京师大学报(自然科学版),2013,36(04):107.
　Zhang Rufeng,Song Jinyuan,Li Peng,et al.Cloning and Characterization of Heat Shock Protein 90 Gene Introns in the Chinese Mitten Crab Eriocheir sinensis[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(04):107.
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中华绒螯蟹热激蛋白90基因内含子的克隆与界定
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年04期
页码:
107
栏目:
生命科学
出版日期:
2013-12-31

文章信息/Info

Title:
Cloning and Characterization of Heat Shock Protein 90 Gene Introns in the Chinese Mitten Crab Eriocheir sinensis
作者:
张入峰; 宋金远; 李 鹏; 陆 舟; 严 洁; 吴 烨; 高 勍
南京师范大学生命科学学院,江苏 南京 210023
Author(s):
Zhang Rufeng; Song Jinyuan; Li Peng; Lu Zhou; Yan Jie; Wu Ye; Gao Qing
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
关键词:
中华绒螯蟹; 热激蛋白90; 内含子; 序列分析
Keywords:
Eriocheir sinensis; heat shock protein 90(HSP90); intron; sequence analysis
分类号:
Q95
文献标志码:
A
摘要:
热激蛋白家族中分子量为90kDa的热激蛋白(HSP90)是真核细胞胞质中最丰富和高度保守的蛋白质之一.HSP90作为一种重要的分子伴侣,广泛参与细胞的激素信号通路、细胞周期调控以及细胞发育等重要的生理过程; 内含子(intron)是基因中的非编码DNA片段,参与基因的组织特异性表达、蛋白质变异、基因转录等多种生物学过程.本文通过PCR扩增得到了中华绒螯蟹(Eriocheir sinensis)HSP90(命名为EsHSP90)的3个内含子序列(分别为296 bp、140 bp和173bp),内含子1和内含子3的剪接符合GT-AG规律,而内含子2不遵循GT-AG或AT-AC规律.EsHSP90的DNA序列全长为3 126 bp.序列比对分析显示EsHSP90与已知甲壳动物的HSP90具有高于80%的序列相似度.
Abstract:
The heat shock protein 90(HSP90)is a highly conserved molecular chaperone particularly important in the maturation of a diverse set of proteins that regulating key steps in various biological processes,such as hormone signaling pathways in cells,cell cycle regulation,cell growth and development,etc..Introns are the noncoding DNA fragments,which are involved in variety of biological activities,such as regulating gene expression,protein alternative splicing and gene transcription,etc..Based on PCR technique,the three introns(296 bp,141 bp and 173 bp)of a HSP90 gene(named as EsHSP90)were identified and characterized from the Chinese mitten crab Eriocheir sinensis.EsHSP90 gene had two GT-AG type introns(intron-1 and intron-3).While exon-intron splicing for intron-2 did not follow the GT-AG or AT-AC rules.The full-length DNA of EsHSP90 is 3,126 bp in size and contains an open reading frame of 2,157 bp which encodes a 718 amino acid polypeptide(82.85 kDa)with HSP90 family signatures and ATP binding domain.Sequence alignment shows that the full-length DNA sequence of EsHSP90 shared more than 80% identity with HSP90 sequences reported in crustaceans.

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备注/Memo

备注/Memo:
收稿日期:2013-07-01.
基金项目:国家自然科学基金(31000954)、教育部高等学校博士点专项研究基金(20103207120007)、江苏省高校自然科学研究项目资助(13KJB180008)、国家基础科学人才培养基金(J1103507)、南京师范大学“大学生实践创新训练计划”项目.
通讯联系人:高 勍,实验师,研究方向:生物学教育.E-mail:gaoqing@njnu.edu.cn
更新日期/Last Update: 2013-12-30