[1]焦东旭,李 娜,程 晨,等.溴氰菊酯诱导果蝇细胞毒性及相关基因表达的变化[J].南京师大学报(自然科学版),2014,37(02):106.
　Jiao Dongxu,Li Na,Cheng Chen,et al.Induction of the Cytotoxicity and the Relative Genes Expression Response of Deltamethrin in Drosophila Kc cells[J].Journal of Nanjing Normal University(Natural Science Edition),2014,37(02):106.
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溴氰菊酯诱导果蝇细胞毒性及相关基因表达的变化()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第37卷
期数:
2014年02期
页码:
106
栏目:
生命科学
出版日期:
2014-06-30

文章信息/Info

Title:
Induction of the Cytotoxicity and the Relative Genes Expression Response of Deltamethrin in Drosophila Kc cells
作者:
焦东旭; 李 娜; 程 晨; 葛春男; 程罗根
南京师范大学生命科学学院,江苏南京210023
Author(s):
Jiao Dongxu; Li Na; Cheng Chen; Ge Chunnan; Cheng Luogen
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
关键词:
溴氰菊酯; 细胞毒性; 实时荧光定量PCR; 免疫印迹分析
Keywords:
deltamethrin; cytotoxicity; real鄄time quantitative PCR; western blotting
分类号:
S481+4
文献标志码:
A
摘要:
溴氰菊酯(deltamethrin,DM)是一种神经毒剂,由于其高效、低毒而被广泛用于农业害虫的防治. 长期大范围不合理的使用导致害虫产生抗药性,但目前其确切的抗药性作用机制仍不十分清楚. 本研究对溴氰菊酯细胞毒性的研究显示DM 染毒可以使果蝇Kc 细胞增殖、细胞形态和细胞活性发生明显变化. 较低和较高浓度的DM 能明显抑制细胞活性,在15 ppm 诱导下果蝇细胞的活性有一定程度的升高. 实时荧光定量PCR 和免疫印迹分析结果显示,经DM 处理后的果蝇Kc 细胞中与氧化应激反应和免疫相关的基因的表达量显著
Abstract:
Deltamethrin(DM)is a kind of nerve agent,and commonly used in controlling crop pests,because of the high efficacy,low toxicity. Widespread and improper use of DM induced insect resistance,but the resistance mechanism is not clear at present. Cytotoxicity analysis reveals that the cells proliferation, cellular morphology and cells viability have significant changes under DM stress. Too Low or too high concentration of DM could obviously inhibit the activity of cells. Under 15 ppm treatment, cell activity was higher. Real-time quantitative PCR and western-blotting analysis demonstrated that the genes associated with oxidative stress and immune responses were induced sharply under DM stress. Our research focuses on the cytotoxicity and gene differential expression,these results can provide basis for the study of molecular toxicology and mechanism of deltamethrin resistance.

参考文献/References:

[1]Casida J E,Gammon D W,Glickman A H,et al. Mechanisms of selective action of pyrethroid insecticides[J]. Annual Review of Pharmacology and Toxicology,1983,23(1):413-438.
[2] 邢剑飞,刘艳,颜冬云. 昆虫对拟除虫菊酯农药的抗性研究进展[J]. 环境科学与技术,2010,33(10):68-74.
[3] Chen X G,Mathur G,James A A. Gene expression studies in mosquitoes[J]. Advances in Genetics,2008,64:19-50.
[4] 戴璇颖,徐世清,陈息林,等. 环境激素阿特拉津对家蚕卵巢培养细胞(BmN)凋亡的影响[J]. 蚕业科学,2006,32(4):495-499.
[5] 方心葵,王朝霞,陆敏,等. 氰戊菊酯对Trichoplusiani 细胞活力的影响[J]. 西北农林科技大学学报:自然科学版,2006,34(4):101-104.
[6] Daborn P J,Yen J L,Bogwitz M R,et al. A single P450 allele associated with insecticide resistance in Drosophila [ J]. Science,2002,297(5 590):2 253-2 256.
[7] Bogwitz M R,Chung H,Magoc L,et al. Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster [J]. Proceedings of the National Academy of Sciences of the United States of America,2005,102(36):12 807-12 812.
[8] Perry T,Batterham P,Daborn P J. The biology of insecticidal activity and resistance[ J]. Insect Biochemistry and Molecular Biology,2011,41(7):411-422.
[9] Tomita T,Scott J G. cDNA and deduced protein sequence of Cyp6D1:the putative gene for a cytochrome p450 responsible for pyrethroid resistance in house fly[J]. Insect Biochemistry and Molecular Biology,1995,25(2):275-283.
[10] 冯国蕾. 神经递3H-去甲肾上腺素释放与家蝇对拟除虫菊酯抗性的关系[J]. 昆虫学报,2002,45(2):204-208.
[11] Misra J R,Horner M A,Lam G,et al. Transcriptional regulation of xenobiotic detoxification in Drosophila[J]. Genes and Development,2011,25(17):1 796-1 806.
[12] Maines M D. The heme oxygenase system:a regulator of second messenger gases[ J]. Annual Review of Pharmacology and Toxicology,1997,37(1):517-554.
[13] Zou S,Meadows S,Sharp L,et al. Genome-wide study of aging and oxidative stress response in Drosophila melanogaster[J].Proceedings of the National Academy of Sciences,2000,97(25):13 726-13 731.
[14] S?derh?ll K,Cerenius L. Role of the prophenoloxidase-activating system in invertebrate immunity[J]. Current Opinion in Im- munology,1998,10(1):23-28.
[15] Gong P, Hu B, Stewart D, et al. Cobalt induces heme oxygenase-1 expression by a hypoxia-inducible factor-independent mechanism in Chinese hamster ovary cells regulation by Nrf2 and MafG transcription factors [ J]. Journal of Biological Chemistry,2001,276(29):27 018-27 025.

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备注/Memo

备注/Memo:
收稿日期:2013-11-28.
基金项目:江苏省高校优势学科(生物学)建设工程项目、江苏省研究生科研创新计划项目、南京师范大学大学生实践创新训练计划项目.
通讯联系人:程罗根,博士,教授,研究方向:分子遗传学,遗传毒理学. E-mail:chengluogen@ njnu. edu. Cn
更新日期/Last Update: 2014-06-30