[1]赵银娟,孙敦平,韩素芬,等.根瘤菌87-1-1诱导刺槐根表皮传递细胞特异性表达的cDNA文库构建及序列分析[J].南京师大学报(自然科学版),2014,37(04):76.
　Zhao Yinjuan,Sun Dunping,Han Sufen,et al.cDNA Library Construction and Analysis of Differentially Expressedin Roots of Robinia pseudoacacia Induced by Rhizobium 87-1-1[J].Journal of Nanjing Normal University(Natural Science Edition),2014,37(04):76.
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根瘤菌87-1-1诱导刺槐根表皮传递细胞特异性表达的cDNA文库构建及序列分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第37卷
期数:
2014年04期
页码:
76
栏目:
生命科学
出版日期:
2014-12-31

文章信息/Info

Title:
cDNA Library Construction and Analysis of Differentially Expressedin Roots of Robinia pseudoacacia Induced by Rhizobium 87-1-1
作者:
赵银娟; 孙敦平; 韩素芬; 吴小芹
南方现代林业协同创新中心,南京林业大学林学院,江苏省有害生物入侵与控制重点实验室,江苏 南京 210037
Author(s):
Zhao Yinjuan; Sun Dunping; Han Sufen; Wu Xiaoqin
Southern China Collaborative Innovation Center of Sustainable Forestry,College of Forestry,Nanjing Forestry University,Jiangsu Key Laboratory for Prevention and Management of Invasion Species,Nanjing 210037,China
关键词:
根瘤菌; 刺槐; 传递细胞; SSH; 功能分析
Keywords:
Rhizobium; Robinia pseudoacacia; transfer cells; SSH; function analysis
分类号:
S718
文献标志码:
A
摘要:
探寻根瘤菌诱导刺槐后根表皮细胞分化成传递细胞的特异性表达基因及其分子机制.采用抑制差减杂交(suppression subtraction hybridization,SSH)技术构建刺槐根系被接种根瘤菌和未被接种根瘤菌二者间的正反两个cDNA文库.各挑选正反文库中500个克隆进行测序,在Blastn、Blastp、SwissProt、KEGG、COG、Interpro 以及Gene ontology(GO)数据库中进行比对注释.结合生理过程对ESTs进行分析,共获得725条非冗余序列(uniEST),正
Abstract:
To find special genes in transfer cells of Locust which was differentiated from root epidemic cells induced by rhizobium,a forward and reverse suppression subtraction hybridization(SSH)cDNA library were constructed successfully.Using cDNA from the roots of Robinia pseudoacacia induced by rhizobium as the tester and cDNA from roots of Locust untreated as driver seperately,suppression subtraction hybridization libraries was constructed.500 colonies in both libraries were isolated and sequenced.Functions of ESTs were analyzed by blast in NCBI including database Nt,Nr,Swissprot,COG,Interpro and GO after removing repeat and redundancy sequences.725 uniESTs including 385 uniESTs in forward library and 340 uniESTs in reverse library were obtained.The assembling provided a total of 51 contigs and 674 singletons by cluster analyses of the uniESTs.Nucleotide homology searched with Blastn in NCBI non-redundant nucleotide database and 674 uniESTs(93% of total uniESTs)were homologous with known genes.Protein homology searched with Blastx in NCBI non-redundant protein database and 648 uinESTs(89% of total uniESTs)were homologous with known proteins.The results of gene ontology(GO)annotation showed that 369 uinESTs were involved in biological process with 270 times,molecular function with 448 times and cell component with 484 times respectively.Among these ESTs,putative proteins were related to posttranslational modification,transcription factors,cell signals,cell wall/cell membrane/endomembrane system,cytoskeleton and nodulin genes in positive library,and related to growth and metabolites biosynthesis in reverse library.These results were generally consistent with physiological processes when the Robinia pseudoacacia were induced by rhizobium.Such as MYB transcription factor,Vesicle-associated membrane protein could be found in positive library which might be special expression in the transfer cells to aid the understanding of development of transfer cells.

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备注/Memo

备注/Memo:
收稿日期:2014-03-26.
基金项目:国家自然科学青年基金(31100448)、高校博士点基金(2113204120004)、江苏省博士后基金.
通讯联系人:吴小芹,博导,教授,研究方向:森林保护及森林有益微生物的研究.E-mail:xqwu@njfu.edu.cn
更新日期/Last Update: 2014-12-31