[1]许剑锋,沈维干,彭琬昕.腺病毒介导的RNA干扰降低VEGF受体FLT-1,KDR转录抑制血管形成[J].南京师大学报(自然科学版),2014,37(04):89.
　Xu Jianfeng,Sheng Weigan,Peng Wanxin.Knockdown of FLT-1,KDR by Adenovirus-Delivered SmallInterfering RNA Inhibits Angiogenesis[J].Journal of Nanjing Normal University(Natural Science Edition),2014,37(04):89.
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腺病毒介导的RNA干扰降低VEGF受体FLT-1,KDR转录抑制血管形成()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第37卷
期数:
2014年04期
页码:
89
栏目:
生命科学
出版日期:
2014-12-31

文章信息/Info

Title:
Knockdown of FLT-1,KDR by Adenovirus-Delivered SmallInterfering RNA Inhibits Angiogenesis
作者:
许剑锋1; 沈维干2; 彭琬昕2
(1.江苏省血液中心,江苏 南京 210042)(2.南京师范大学生命科学学院,江苏 南京 210023)
Author(s):
Xu Jianfeng1; Sheng Weigan2; Peng Wanxin2
(1.Jiangsu Province Blood Center,Nanjing 210042,China)(2.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
关键词:
血管生成; 血管内皮生长因子受体; RNA干扰
Keywords:
angiogenesis; FLT-1; KDR; RNAi
分类号:
Q291
文献标志码:
A
摘要:
本研究用细菌内同源重组的方法构建了针对VEGF受体FLT-1,KDR的siRNA表达重组腺病毒AdH1-siRNA/FLT-1和AdH1-siRNA/KDR,感染HUVEC细胞,观察两种病毒对HUVEC细胞体外增殖和形成微血管的干扰作用.通过RT-PCR实验表明,AdH1-siRNA/FLT-1和AdH1-siRNA/KDR均可特异性地下调血管内皮细胞(HUVEC)中的FLT-1mRNA和KDR mRNA水平.与对照组相比,FLT-1的mRNA水平下降为40%,KDR的mRNA水平下降为52%.AdH1-
Abstract:
This study used the homologous recombination technique constructed a recombinant adenovirus AdH1-siRNA/FLT-1 and AdH1-siRNA/KDR targeting FLT-1 and KDR infects human endothelial cells(HUVEC),observation the effect of the adenovirus interfere the proliferation of HUVEC in vitro and the tube formation.Reverse transcription-PCR showed that this adenovirus could specifically decrease level of the FLT-1 and KDR mRNA of HUVEC.Compared with control group,the FLT-1 and KDR mRNA expression of decreased to 40% and 52%.AdH1-siRNA/FLT-1 and KDR can efficiently interfere the proliferation of HUVEC,9 d after infection of adenovirus,the average cell number of control group and vector group are 31.5×104/μL and 28×104/μL,that of groups respectively infected with AdH1-siRNA/VEGF are 21.5×104/μL,and can efficiently interfere the tube formation of HUVEC on matrigel.Averagely,the tube number of every HPF is 10.75 in the control group,and 10.25 in the vector group.In the group treated with AdH1-siRNA/VEGF,there are 7 tubes in the HPE.It is sowed that AdH1-siRNA/FLT-1 and AdH1-siRNA/KDR can also interfere tube formation.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-06-30.
通讯联系人:许剑峰,硕士,研究方向:细胞生物学.E-mail:xjf646566@sina.com
更新日期/Last Update: 2014-12-31