[1]彭琬昕,万燕雅,葛璐,等.siRNA干扰即刻早期基因Egr-1对汉防己甲素诱导的胰腺癌细胞自噬、凋亡的作用研究[J].南京师范大学学报(自然科学版),2015,38(03):71.
PengWanxin,WanYanya,GeLu,et al.EffectofRNAInterferenceTargetingEgr-1InhibiteTetrandrine-InducedAutophagyandApoptosisofPancreaticPaTu8988Cellsinvitro[J].Journal of Nanjing Normal University(Natural Science Edition),2015,38(03):71.
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siRNA干扰即刻早期基因Egr-1对汉防己甲素诱导的胰腺癌细胞自噬、凋亡的作用研究()
《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]
- 卷:
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第38卷
- 期数:
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2015年03期
- 页码:
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71
- 栏目:
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生命科学
- 出版日期:
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2015-09-30
文章信息/Info
- Title:
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EffectofRNAInterferenceTargetingEgr-1InhibiteTetrandrine-InducedAutophagyandApoptosisofPancreaticPaTu8988Cellsinvitro
- 作者:
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彭琬昕1; 万燕雅2; 葛璐1; 金洁1; 龚爱华1; 吴朝阳2
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(1.江苏大学医学院生物系,江苏镇江212013)
(2.江苏大学附属人民医院肿瘤放疗科,江苏镇江212013)
- Author(s):
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PengWanxin1; WanYanya2; GeLu1; JinJie1; GongAihua1; WuChaoyang2
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(1.DepartmentofBiology,SchoolofMedicine,JiangsuUniversity,Zhenjiang212013,China)
(2.DepartmentofRadiationOncology,AffiliatedPeople’sHospital,JiangsuUniversity,Zhenjiang212013,China)
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- 关键词:
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胰腺癌; 汉防己甲素; Egr-1; 细胞自噬; 细胞凋亡
- Keywords:
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pancreaticcancer; tetrandrine; Egr-1; autophagy; cellapoptosis
- 分类号:
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R730.5
- 文献标志码:
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A
- 摘要:
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本实验将胰腺癌细胞PaTu8988分别转染无关干扰对照组(siRNA-Scramble)和干扰即刻早期基因Egr-1组(siRNA-Egr-1),再用不同浓度的汉防己甲素处理24h后,CCK-8法检测不同浓度的汉防己甲素对细胞活力的影响;Westernblot检测细胞自噬和凋亡相关蛋白表达水平的改变.结果表明:(1)低浓度的汉防己甲素能显著激活PaTu8988细胞自噬,但对增殖和凋亡无明显影响;(2)与对照组相比,转染了siRNA-Egr-1组自噬相关蛋白Beclin-1表达量显著下降,LC3II/LC3I比值下调;凋亡相关蛋白Bcl2/Bax的比值降低.上述结果表明,干扰即刻早期基因Egr-1的表达可以有效抑制低浓度汉防己甲素引起的胰腺癌细胞PaTu8988自噬,促进细胞凋亡.
- Abstract:
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HumanpancreaticcancercellsPaTu8988weretransfectedwithsiRNA-ScrambleandsiRNA-Egr-1for24hrespectively,thentreatedwithtetrandrineforanother24h.Treatedbydifferentconcentrationoftetrandrine,thecytotoxiceffectoftetrandrinewasexaminedbyCCK-8,andexpressionofautophagy-relatedproteinweredetectedbyWesternblot.Theexpressionofcellautophagy-andapoptosis-relatedproteinweredetectedbyWesternblot.Alloftheresultsindicatedthatcomparingwithcontrolgroup,siEgr-1significantlyattenuatestetrandrine-inducedautophagyandpromotestheapoptosisofPaTu8988cells.
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备注/Memo
- 备注/Memo:
-
收稿日期:2014-10-05.
基金项目:国家自然科学基金(31100964)、江苏省博士后基金(1402102C)、江苏大学高级人才科研启动基金(10JDG045).
通讯联系人:吴朝阳,博士,主任医师,研究方向:肿瘤耐药时间.E-mail:wuchaoyang9@163.com
更新日期/Last Update:
2015-09-30