[1]宋婷婷,尹慧慧,杨 帆,等.一株产CGTase环糊精葡萄糖基转移酶的细菌改性甜菊苷的研究[J].南京师范大学学报(自然科学版),2017,40(01):95.[doi:10.3969/j.issn.1001-4616.2017.01.014]
　Song Tingting,Yin Huihui,Yang Fan,et al.Bioconversion of Stevioside by CGT7 Bacteriawith Cyclodextrin Glucanotransferase Activity[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(01):95.[doi:10.3969/j.issn.1001-4616.2017.01.014]
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一株产CGTase环糊精葡萄糖基转移酶的细菌改性甜菊苷的研究()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年01期
页码:
95
栏目:
·数学与计算机科学·
出版日期:
2017-03-31

文章信息/Info

Title:
Bioconversion of Stevioside by CGT7 Bacteriawith Cyclodextrin Glucanotransferase Activity
文章编号:
1001-4616(2017)01-0095-04
作者:
宋婷婷1; 尹慧慧1; 杨 帆2; 闫 莽1; 陈育如1; 2
(1.南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省功能微生物与基因组学重点实验室,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023)(2.格里菲斯大学环境学院和环境未来研究所,澳大利亚 昆士兰 QLD 4222)
Author(s):
Song Tingting1; Yin Huihui1; Yang Fan2; Yan Mang1; Chen Yuru1; 2
(1.Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Functional Microbes and Genomic,Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing
关键词:
环糊精葡萄糖基转移酶; 甜菊糖; 酶法改性; 糖基供体; 酶活性
Keywords:
cyclodextrin glucanotransferase; steviol glycoside; enzymatic modification; glycosyl donor; enzyme activity
分类号:
Q814
DOI:
10.3969/j.issn.1001-4616.2017.01.014
文献标志码:
A
摘要:
本文对筛选的一株产CGTase环糊精葡萄糖基转移酶的细菌(Bacillus sp. CGT7)转化并改性甜菊苷进行了研究,探讨了影响酶活性和转化效果的因素. 实验结果表明,在CGT7菌的培养过程中,碳源、氮源、温度和初始pH等因素对菌株产酶活性均有不同程度的影响,适宜的碳源为马铃薯淀粉,氮源为豆粕粉,培养温度为30 ℃,初始pH值为8.5. 适宜条件下培养的CGT7菌的酶活性可达544 U/mL,以甜菊糖苷为底物、环糊精为糖基供体时,转糖苷产物Stv-Glu浓度为0.61 mg/mL.
Abstract:
This work screened the strain CGT7 which produces cyclodextrin glucanotransferase,and studied its bioconversion and modification activities from stevioside into Stv-Glu. The efficient factors were discussed. Experimental results showed that carbon source,nitrogen source,temperature and pH value of culture medium can effect on cyclodextrin glucanotransferase activity of CGT7 observably. Potato starch and soybean cake meal are the appropriate carbon and nitrogen sources with an initial pH value of 8.5 at 30 ℃. The cyclodextrin glucanotransferase activity of CGT7 culture medium reached 544 U/mL under the optimized condition,and the concentration of biocoversion product Stv-Glu was obtained at 0.61 mg/mL when stevioside was introduced as the substrate and cyclodextrin as the glycosyl donor.

参考文献/References:

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相似文献/References:

[1]陈育如,杨凤平,杨帆,等.甜叶菊及甜菊糖的多效功能与保健应用[J].南京师范大学学报(自然科学版),2016,39(02):56.[doi:10.3969/j.issn.1001-4616.2016.02.011]
　Chen Yuru,Yang Fengping,Yang Fan,et al.The Functions of Health Care and Application ofStevia rebaudiana and Stevioside[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(01):56.[doi:10.3969/j.issn.1001-4616.2016.02.011]

备注/Memo

备注/Memo:
收稿日期:2016-03-23.
基金项目:江苏省高校自然科学研究重大项目(15KJA210002)、江苏省“六大人才高峰”高层次人才选拔培养项目(NY-011-2015).
通讯联系人:陈育如,教授,博士,研究方向:天然产物与药物研究. E-mail:chenyuru@njnu.edu.cn
更新日期/Last Update: 1900-01-01