[1]叶 春.长期游泳运动对高脂饲料喂养大鼠肝脏中CYP7A1及D-双功能蛋白表达的影响[J].南京师范大学学报(自然科学版),2018,41(01):112.[doi:10.3969/j.issn.1001-4616.2018.01.020]
　Ye Chun.Long-term Swimming Enhance the Expression of D-bifunctionalProtein and CYP7A1 in Rats Liver of High-fat Diet[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(01):112.[doi:10.3969/j.issn.1001-4616.2018.01.020]
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长期游泳运动对高脂饲料喂养大鼠肝脏中CYP7A1及D-双功能蛋白表达的影响()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年01期
页码:
112
栏目:
·生命科学·
出版日期:
2018-03-31

文章信息/Info

Title:
Long-term Swimming Enhance the Expression of D-bifunctionalProtein and CYP7A1 in Rats Liver of High-fat Diet
文章编号:
1001-4616(2018)01-0112-04
作者:
叶 春
南京师范大学体育科学学院,江苏 南京 210023
Author(s):
Ye Chun
School of Sport Sciences,Nanjing Normal University,Nanjing 210023,China
关键词:
游泳运动; 高脂饲料; CYP7A1; DBP
Keywords:
swimming; high fat diet; CYP7A1; DBP
分类号:
G804.5
DOI:
10.3969/j.issn.1001-4616.2018.01.020
文献标志码:
A
摘要:
探讨长期耐力运动对高脂饲料喂养的大鼠胆汁酸生成关键酶CYP7A1和D-双功能蛋白(DBP)表达的影响. 30只大鼠随机分为高脂组(HC)、高脂运动组(HE)、正常组(C). 高脂组和高脂运动组采用高脂饲料喂养. 高脂运动组喂养4周后,进行16周的游泳运动. 20周后,测定三组大鼠肝脏中CYP7A1和DBP的表达,血清中生化指标的变化以及粪便中的胆汁酸,并取肝脏作病理切片分析. 测定结果显示,高脂运动组较高脂组肝脏的脂肪变性明显好转,血清中chol、TG、LDL、CP均趋向正常; 高脂运动组肝脏中CYP7A
Abstract:
To detect the effect of long term swimming on bile acid classic synthesis pathway in high fat diet rats. Thirty healthy male SD rats were randomly and equally divided into following 3 groups,normal control group(C,fed with standard diet),high fat diet fed control group(HC)and long-term exercise(HE). The rats in group CON were fed in diet control groups and fed with the standard diet. The rats in group HC were continuously fed with high-fat diet without exercise. The rats in group HC were fed with high-fat diet without exercise for 4 weeks,then with 90 min unloaded swimming 5 times per week. The whole duration of the study were 20 weeks,the serum concentration of Glu、Chol、TG、HDL、LDL、CP,and fecal bile acids were assayed. The body and liver weight of rats were recorded. The mRNA levels of hepatic DBP and CYP7A1 were detected by RT-PCR. Serum Glu、TG、chol and LDL were lower in HE group compared HC group(P<0.05). The expression of CYP7A1 and DBP were up-regulation after long-term swimming. The expression of CYP7A1 and DBP could be up-regulated by long-term swimming,thus effectively increasing bile acid excreting and improving the disorders of lipid metabolism.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-10-15.
基金项目:江苏省高校自然科学研究面上专案(09KJB34001).
通讯联系人:叶春,博士,讲师,研究方向:运动生化. E-mail:12144@njnu.edu.cn
更新日期/Last Update: 2018-03-31