[1]王丽平,余 群,翁锡全,等.间歇低氧暴露对逐步递增负荷运动大鼠白细胞及其分类计数的影响[J].南京师范大学学报(自然科学版),2018,41(01):116.[doi:10.3969/j.issn.1001-4616.2018.01.021]
　Wang Liping,Yu Qun,Weng Xiquan,et al.Effects of Intermittent Hypoxic Exposure on WBC Counting andClassification of Gradually Increasing Load Exercise SD Rats[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(01):116.[doi:10.3969/j.issn.1001-4616.2018.01.021]
点击复制

间歇低氧暴露对逐步递增负荷运动大鼠白细胞及其分类计数的影响()
分享到：

《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年01期
页码:
116
栏目:
·生命科学·
出版日期:
2018-03-31

文章信息/Info

Title:
Effects of Intermittent Hypoxic Exposure on WBC Counting andClassification of Gradually Increasing Load Exercise SD Rats
文章编号:
1001-4616(2018)01-0116-05
作者:
王丽平1; 余 群1; 翁锡全2; 林文弢2
(1.安顺学院体育学院,贵州 安顺 561000)(2.广州体育学院运动生物化学重点实验室,广东 广州 510075)
Author(s):
Wang Liping1; Yu Qun1; Weng Xiquan2; Lin Wentao2
(1.Anshun University,Anshun 561000,China)(2.Guangzhou Sport University,Guangzhou 510075,China)
关键词:
递增负荷运动; 运动性低血红蛋白; 间歇低氧; 白细胞; 动物实验
Keywords:
increasing load exercise; exercise-induced lower hemoglobin; intermittent hypoxic; WBC; animal experiment
分类号:
G886
DOI:
10.3969/j.issn.1001-4616.2018.01.021
文献标志码:
A
摘要:
低氧训练是体育训练项目中广泛采用并行之有效的运动训练方法和手段. 在低氧训练的过程中,机体处于低氧环境和运动的双重刺激下,对外周血中的免疫细胞参数有明显的影响. 本文将探讨间歇低氧刺激对逐步递增负荷运动大鼠白细胞的影响. 将大鼠随机分为安静组、运动组、运动+低氧连续暴露组和运动+间歇低氧暴露组. 后3组进行6周递增负荷跑台运动,运动+低氧连续暴露组和运动+间歇低氧暴露组从第4周起各在运动后进行人工低氧(O2含量为14.5%)2 h连续暴露和间隔3 h的暴露. 6周训练后,运动组大鼠运
Abstract:
This paper discusses the intermittent hypoxia stimulate WBC(White Blood Cell)of gradually increasing load exercise SD rats. It explores the changes of the body’s nonspecific immunity when hypoxia prevents movement lower hemoglobin and provides a reference for practical training and people fitness. 35 SD rats were divided into four groups:control group(A),training group(B),hypoxic exposure 2 hours group(C)and hypoxic exposure(1+1)hours group(D). The rats of training groups were given 6-week treadmill exercise with progressive loading. Three of the training groups were put in artificial hypoxic environment(14.5% O2)1 hour,2 hours and(1+1)hour per day respectively for three weeks. In the end of the six week,we tested the levels of the red blood cells and white blood cells parameters. After six-week progressive treadmill exercise training,the phenomenon of lower hemoglobin took place. The WBC of the rats in the training groups were significantly lower than that of the control group(P<0.05,P<0.01),but it did not significantly changes in the intermittent hypoxic exposure groups comparing with the training groups(P>0.05). The Neut and Lymph in the training groups were significantly or very significantly lower than the control group(P<0.05 or P<0.01),the Mono in hypoxic exposure(1+1)hour group were significantly lower than the control group,the Aacidophil and Basophil had no significant difference between the four groups(P>0.05). Repeated small amounts of hypoxic exposure can be effective prevention and treatment of exercise-induced lower hemoglobin phenomenon,but it has a large effect on the body’s immune. We should be careful to choose suitable way of hypoxic exposure in hypoxia training practice.

参考文献/References:

[1] LEVINE B D,STRAY G J. A practical approach o altitude training:where to live and training for optimal performance enhancement[J]. Int J Sports Med,1992(suppl 1):s209-s212.
[2]CALDWELL C C,KOJIMA H,LUKASHEV D,et al. Differential effects of physiologically relevant hypoxic conditions on T lympho-cyte development and effectors functions[J]. J Immunol,2001,167(11):6 140-6 149.
[3]王竹影,姜文凯,陈龙孙,等. 中、高强度不同模式低氧训练对大鼠白细胞及其分类计数的影响[J]. 南京师大学报(自然科学版),2011,34(4):147-147.
[4]余群,翁锡全,林文弢,等. 间歇低氧预防SD大鼠大运动负荷训练期血红蛋白下降及其机制探讨[J]. 中国运动医学杂志,2010,29(4):399-402.
[5]张漓,路瑛丽,徐建方,等. 低氧耐力运动4周内大鼠ET-1与NO生成的时序性变化[J]. 中国体育科技,2014,50(4):108-116.
[6]YAMAMURA C,ZUSHI S,TAKATA K,et al. Physiological characteristics of well-trained synchronized swimmers in relation to performance scores[J]. Int J Sports Med,1999,20(4):246-251.
[7]WANG J S,WENG T P. Hypoxic exercise training promotes antitumour cytotoxicity of natural killer cells in young men[J]. Clinical science,2011,121(8):343-353.
[8]PE DERS EN B K,HOFFM AN-GOET Z L. Exercise and the immune system. regulation,integration and adaption[J]. Phys iol Rev,2000,80:1 055-1 081.
[9]ISOWA T,OHIRA H,MURASHIMA S. Reactivity of immune,endocrine and cardiovascular parameters to active and passive a-cute stress[J]. Biol Psychol,2004,65(2):101-120.
[10]PE DERSEN BK,S TEE SBERG A. Ex ercise and Hypoxia:Effects on Leukocytes and Interleuk in-6-shared M echanism s[J]. Med Sci Sports Exe,2002,34(12):2004-13.
[11]王竹影,姜文凯,陈龙,等. 中、高强度不同模式低氧训练对大鼠白细胞及其分类计数的影响[J]. 南京师大学报(自然科学版),2011,34(4):142-147.
[12]TURNER L,SCOTTON C,NEGUS R,et al. Hypoxia inhibits macrophage migration[J]. Eur J Immunol,1999,29(7):2 280-2 287.
[13]NIESS A M,FEHRENBACH E,STROBEL G. Evaluation of stress responses to interval training at low and moderate altitudes[J]. Med Sci Sports Exerc,2003,35(2):263-269.
[14]FISCHETTI F,FABRIS B,ZACEARIA M,et al. Effects of prolonged high-altitude exposure on peripheral adrenergic receptor in young healthy volunteers[J]. Eur J Appl Physiol,2000,82(5):439-445.
[15]常芸,何子红,王莱芮,等. 高原训练对国家短道速滑运动员细胞免疫功能的影响[J]. 体育科学,2002,22(1):86-89.
[16]TIOLLIER E,SCHMITT L,BURNAT P,et al. Living high-training low altitude training:effects on mucosal immunity[J]. European journal of applied physiology,2005,94(3):298-304.
[17]CARR A J,SAUNDERS P U,VALLANCE B S,et al. Increased hypoxic dose after training at low altitude with 9h per night at 3 000 m normobaric hypoxia[J]. Journal of sports science and medicine,2015 Dec; 14(4):776-782.
[18]赵杰修,张漓,路瑛丽,等. 高原(低氧)和高温环境下运动训练生理生化监控研究进展[J]. 中国运动医学杂志,2016(12):1 165-1 171.
[19]NIESS A M,FEHRENBACH E,STROBEL G. Evaluation of stress responses to interval training at low and moderate altitudes[J]. Med Sci Sports Exerc,2003,35(2):263-269.
[20]GOSWAMI A R,DUTTA G,GHOSH T. Effects of vitamin C on the hypobaric hypoxia-induced immune changes in male rats[J]. Int J Biometeorol,2014 Nov; 58(9):1 961-1 971.

备注/Memo

备注/Memo:
收稿日期:2017-04-18.
基金项目:贵州省科学技术基金项目(黔科合J字LKA[2013]07号).
通讯联系人:王丽平,硕士,副教授,研究方向:运动训练生理生化原理、网球训练. E-mail:447568832@qq.com
更新日期/Last Update: 2018-03-31