[1]尹慧慧,陈育如,赵文文.冠突散囊菌利用不同原料产洛伐他汀的条件优化[J].南京师范大学学报(自然科学版),2018,41(02):93.[doi:10.3969/j.issn.1001-4616.2018.02.017]
　Yin Huihui,Chen Yuru,Zhao Wenwen.Optimization of Producing Lovastatin by Eurotiumcristatum with Different Materials[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(02):93.[doi:10.3969/j.issn.1001-4616.2018.02.017]
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冠突散囊菌利用不同原料产洛伐他汀的条件优化()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年02期
页码:
93
栏目:
·生命科学·
出版日期:
2018-06-30

文章信息/Info

Title:
Optimization of Producing Lovastatin by Eurotiumcristatum with Different Materials
文章编号:
1001-4616(2018)02-0093-06
作者:
尹慧慧; 陈育如; 赵文文
南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与基因组学重点实验室,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023
Author(s):
Yin Huihui; Chen Yuru; Zhao Wenwen
School of Life Sciences,Nanjing Normal University,Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Jiangsu Key Laboratory for Microbes and Functional Genomic,Nanjing 21
关键词:
冠突散囊菌; 洛伐他汀; 维生素B2; 甜菜粉; 甜叶菊
Keywords:
Eurotium cristatum; lovastatin; vitamin B2; beet power; stevia rebaudiana
分类号:
Q935
DOI:
10.3969/j.issn.1001-4616.2018.02.017
文献标志码:
A
摘要:
本研究以甜菜粉、甜菜粕、甜叶菊、甘草渣及麸皮为原料用冠突散囊菌发酵产洛伐他汀,分别考察了这些原料和不同因素对洛伐他汀产量的影响. 甜菜粉作为培养基时洛伐他汀的含量最高为95 mg/kg. 添加0.15%维生素B2以甜菜粉为原料产洛伐他汀的量最高(134 mg/kg),与不添加维生素B2相比产量提高了41%,以添加量(A)、发酵温度(B)、发酵时间(C)及接种量(D)4个因素进行正交试验. 结果表明,各因素对洛伐他汀产量的影响大小依次为:添加量(A)>接种量(D)>发酵时间(C)>发酵温度(B). 最优组合为A2B3C1D2,在此条件下洛伐他汀产量为146.4 mg/kg.
Abstract:
In this work,it is studied on the production of lovastatin by Eurotium cristatum from beet powder,beet pulp,stvia rebaudiana,glycyrrhiza residue and wheat bran as raw materials,and the effects of these materials and different factors on the yield of lovastatin were investigated. Eurotium cristatum which cultivated by beet powder had the highest lovastatin level(95 mg/kg). Vitamin B2(with a addition of 0.15%)could make the production of lovastatin significantly raise 41%(134 mg/kg)compared with no precursors. Choose 4 factors to conduct orthogonal test,the results showed the influence order of all factors are,precursors concentration(A)>inoculum concentration(D)>fermentation time(C)>fermentation temperature(B). The optimum extraction technology is A2B3C1D2,and the production of lovastatin could reach 146.4 mg/kg.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-07-10.
基金项目:江苏省高校自然科学研究重大项目(15KJA210002)、省“六大人才高峰”高层次人才选拔培养项目(NY-011-2015).
通讯联系人:陈育如,教授,博士,研究方向:天然产物与药物研究. E-mail:chenyuru@njnu.edu.cn
更新日期/Last Update: 2018-11-06