[1]薛 藩,韦慧仙,胡珈玮,等.基因编辑方法研究进展——以大肠杆菌基因敲除方法为例[J].南京师范大学学报(自然科学版),2018,41(03):102.[doi:10.3969/j.issn.1001-4616.2018.03.016]
　Xue Fan,Wei Huixian,Hu Jiawei,et al.Progress in Gene Editing Methods—Taking the E.coli Gene Knockout Method as an Example[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(03):102.[doi:10.3969/j.issn.1001-4616.2018.03.016]
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基因编辑方法研究进展——以大肠杆菌基因敲除方法为例()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年03期
页码:
102
栏目:
·生命科学·
出版日期:
2018-09-30

文章信息/Info

Title:
Progress in Gene Editing Methods—Taking the E.coli Gene Knockout Method as an Example
文章编号:
1001-4616(2018)03-0102-07
作者:
薛 藩; 韦慧仙; 胡珈玮; 王进军
扬州大学环境科学与工程学院,江苏 扬州 225127
Author(s):
Xue Fan; Wei Huixian; Hu Jiawei; Wang Jinjun
College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China
关键词:
大肠杆菌; 基因修饰; Red同源重组; CRISPR/Cas技术
Keywords:
Escherichia coli; gene modification; red homologous recombination; CRISPR/Cas technology
分类号:
Q348
DOI:
10.3969/j.issn.1001-4616.2018.03.016
文献标志码:
A
摘要:
大肠杆菌是分子生物学的重要研究对象,是生产氨基酸、有机酸和重组蛋白等物质的主要微生物. 在分子生物实验中,常常不需要完整的大肠杆菌基因序列,这时如何截取所需要的基因序列就成了值得研究的问题. Red同源重组是大肠杆菌基因敲除的传统方法,主要利用自身的RecA同源重组系统编码中RecA和RecBCD蛋白,介导DNA进行同源重组. 几经技术革新,但该系统仍然存在很多不足. 基因组编辑三大技术是TALEN、ZFN和CRISPR/Cas,其中的CRISPR/Cas技术更是当今世界上最具发展前景的革命性基因修饰技术.
Abstract:
Escherichia coli is an important research object of molecular biology,it is the main microbe who can produce amino acids,organic acids and recombinant proteins and other substances. In molecular biology experiments,we do not need a complete sequence of Escherichia coli gene,then how to intercept the gene sequence become worthy of study. Red homologous recombination is the traditional method of gene knockout of Escherichia coli. The RecA and RecBCD proteins are mainly encoded by RecA homologous recombination system,which mediates DNA for homologous recombination. After several technological innovation,there are still many deficiencies. Genomics editing includes the three major technologies,TALEN,ZFN and CRISPR/Cas. One of the CRISPR/Cas technology is the world’s most promising revolutionary genetic modification technology.

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[1]李东霞,杨振,潘少坤,等.原核表达系统pET28a(+)中抗CD20微抗体的构建和表达[J].南京师范大学学报(自然科学版),2012,35(03):74.
　Li Dongxia,Yang Zhen,Pan Shaokun,et al.Construction and Expression of Anti-CD20 Minibody in pET28a(+) of Prokaryotic Expression System[J].Journal of Nanjing Normal University(Natural Science Edition),2012,35(03):74.

备注/Memo

备注/Memo:
收稿日期:2017-10-24.
基金项目:教育部留学回国人员科研启动基金(20151098)、扬州市基础研究计划(自然科学基金)-面上项目(YZ2015098)、扬州大学高层次人才科研启动基金、扬州大学大学生科技创新基金.
通讯联系人:王进军,副教授,研究方向:环境微生物学. E-mail:wangjinjun@yzu.edu.cn
更新日期/Last Update: 2018-11-19