[1]陈铭月,王飞飞,李 鹏,等.多疣壁虎胚胎发育过程中角蛋白的表达分析[J].南京师范大学学报(自然科学版),2019,42(02):99-104.[doi:10.3969/j.issn.1001-4616.2019.02.016]
　Chen Mingyue,Wang Feifei,Li Peng,et al.The mRNA Expression of Keratin Genes in EmbryonicDevelopment of Gekko japonicus[J].Journal of Nanjing Normal University(Natural Science Edition),2019,42(02):99-104.[doi:10.3969/j.issn.1001-4616.2019.02.016]
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多疣壁虎胚胎发育过程中角蛋白的表达分析()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第42卷
期数:
2019年02期
页码:
99-104
栏目:
·生命科学·
出版日期:
2019-06-30

文章信息/Info

Title:
The mRNA Expression of Keratin Genes in EmbryonicDevelopment of Gekko japonicus
文章编号:
1001-4616(2019)02-0099-06
作者:
陈铭月; 王飞飞; 李 鹏; 严 洁; 周开亚
南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,江苏 南京 210023
Author(s):
Chen Mingyue; Wang Feifei; Li Peng; Yan Jie; Zhou Kaiya
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210023,China
关键词:
多疣壁虎; α角蛋白; β角蛋白; mRNA表达
Keywords:
Gekko japonicus; α-keraton; β-keraton; mRNA expression
分类号:
Q951+3
DOI:
10.3969/j.issn.1001-4616.2019.02.016
文献标志码:
A
摘要:
角蛋白包括α角蛋白和β角蛋白两种类型. 迄今α角蛋白被发现存在于所有的脊椎动物中,而β角蛋白仅存在于鸟类和爬行类表皮及其附属物中. 本研究通过同源搜索比对鉴定了多疣壁虎(Gekko japonicus)的 1个 β角蛋白基因:G.japonicus claw keratin-like(LOC107121854),暂命名为ge-gprp-13,和1个α角蛋白基因:G.japonicus keratinocyte associated protein 2(KRTCAP2),暂命名为ge-krtcap2. 利用荧光定量PCR方法比较分析了多疣壁虎ge-gprp-13和ge-krtcap2基因在其胚胎发育过程中的mRNA表达模式. 研究结果表明,在多疣壁虎胚胎发育的不同时期,α角蛋白基因(ge-krtcap2)和β角蛋白基因(ge-gprp-13)的表达模式不同:在趾部发育的初期,α角蛋白基因先表达,而β角蛋白基因主要在趾部发育的后期进行表达,并且在趾部形成的整个过程中,β角蛋白基因的表达量远远高于α角蛋白基因的表达量.
Abstract:
Reptile epidermis contains two types of keratins—α-keratins and β-keratins. Up to date,α-keratins are reported to be present in all vertebrates,while β-keratins are only found in birds and reptiles. In the present study,two keratin genes of Gekko japonicus were identified from the National Center of Biotechnology Information database,one β-keratin gene,G.japonicus claw keratin-like(LOC107121854)tentatively named ge-gprp-13 and one α-keratin gene,G.japonicus keratinocyte associated protein 2(KRTCAP2)tentatively named ge-krtcap2. The mRNA expression of keratin genes during embryonic development of the gecko was studied by using fluorescence quantitative real-time PCR. The results showed that the α-keratin gene(ge-krtcap2)and the β-keratin gene(ge-gprp-13)have different expression patterns in different stages of embryonic development of the gecko. In the early stage of toe development,the α-keratin gene is expressed first,while the β-keratin gene is mainly expressed in the later stage of toe development. And during the formation of the toe,the expression level of the β-keratin gene is much higher than the α-keratin gene.

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备注/Memo

备注/Memo:
收稿日期:2019-01-08.
基金项目:国家自然科学基金资助项目(31672269)、江苏省高校优势学科Ⅱ期项目(PAPD)、江苏省高等学校自然科学研究面上项目(17KJD240001)、江苏高校品牌专业建设工程一期项目-生物科学(PPZY2015B117).
通讯联系人:严洁,副教授,研究方向:动物遗传与系统地理. E-mail:janeiam@163.com
更新日期/Last Update: 2019-06-30