[1]郑丽蓉,许艳华,曾卫卫,等.细胞膜去极化通过激活MAPK信号通路促进原代肌母细胞增殖[J].南京师范大学学报(自然科学版),2020,43(01):100-106.[doi:10.3969/j.issn.1001-4616.2020.01.015]
 ZhengLirong,XuYanhua,ZengWeiwei,et al.PlasmaMembraneDepolarizationPromotesPrimaryMyoblastProliferationThroughActivationofMAPKSignalingPathway[J].JournalofNanjingNormalUniversity(NaturalScienceEdition),2020,43(01):100-106.[doi:10.3969/j.issn.1001-4616.2020.01.015]
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细胞膜去极化通过激活MAPK信号通路促进原代肌母细胞增殖()
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《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第43卷
期数:
2020年01期
页码:
100-106
栏目:
·生物学·
出版日期:
2020-03-15

文章信息/Info

Title:
PlasmaMembraneDepolarizationPromotesPrimaryMyoblastProliferationThroughActivationofMAPKSignalingPathway
文章编号:
1001-4616(2020)01-0100-07
作者:
郑丽蓉许艳华曾卫卫陈宇琛范春娥陈华群
南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,江苏南京210023
Author(s):
ZhengLirongXuYanhuaZengWeiweiChenYuchenFanChun’eChenHuaqun
SchoolofLifeSciences,NanjingNormalUniversity,JiangsuKeyLaboratoryforMolecularandMedicalBiotechnology,Nanjing2l0023,China
关键词:
去极化肌母细胞细胞增殖MAPK信号通路
Keywords:
depolarizationprimarymyoblastproliferationMAPKsignalingpathway
分类号:
Q291
DOI:
10.3969/j.issn.1001-4616.2020.01.015
文献标志码:
A
摘要:
细胞膜去极化可促进某些细胞包括肌卫星细胞的增殖,但去极化对于肌母细胞的作用还不清楚.本文通过培养基中高浓度的钾离子(K+)引发小鼠原代肌母细胞膜的去极化,采用直接计数法评估细胞的增殖能力.结果显示,在K+浓度为25mmol/L的培养基中培养24h后,肌母细胞数量较常规培养基(5mmol/LK+)明显增加,但K+高至50mmol/L和100mmol/L时对细胞增殖没有促进作用.通过流式细胞技术进行细胞周期分析显示,25mmol/LK+处理使G1期的细胞数量减少,S期的细胞数量增加.免疫荧光染色和Westernblot研究发现,25mmol/LK+处理后,对肌母细胞的肌分化能力没有影响.进一步研究发现,25mmol/LK+明显促进了肌母细胞MAPK信号通路(ERK1/2)的激活,并与其促进细胞增殖的效应相关.MEK1/ERK1/2的两种抑制剂U0126和PD98059均可减弱细胞膜去极化引起的细胞增殖促进效应.综上,研究结果表明细胞膜去极化通过激活MAPK通路促进小鼠原代肌母细胞的增殖.
Abstract:
Plasmamembrane(PM)depolarizationcanenhancetheproliferationofcertaintypesofcellsincludingsatellitecell.However,theeffectsofPMdepolarizationontheproliferationofmyoblasthavenotbeendetermined.Inthisstudy,itwasinvestigatedtheeffectsofPMdepolarizationviahighconcentrationofextracellularpotassiumion(K+)ontheproliferationofmurineprimarymyoblasts.Bydirectcellcounting,itwasshownanapparentincreaseinthenumberofcellsculturedinmediumcontaining25mmol/LK+comparedwiththatofregularmedium(5mmol/LK+).However,excessivelyhigher(50mmol/Land100mmol/L)concentrationsofK+didnotpromotethegrowthofmyoblasts.Inaddition,adecreaseinthenumberofcellsundergoingG1phaseandanincreaseinthatofSphasewasdetectedbyFACStechnology.ImmunostainingandWesternblotanalysisshowedthatthemyogenicdifferentiationpotentialwasnotalteredafterPMdepolarizationtreatment.Furthermore,itwasfoundthatthebasallevelofactivatedextracellularsignal-regulatedkinase1/2(ERK1/2)wasenhancedwithPMdepolarizationtreatment.BothU0126andPD98059,theMEK1/ERK1/2inhibitors,obviouslyattenuatedtheproliferationenhancementofthecells.Takentogether,ourdataindicatethatPMdepolarizationpromotestheproliferationofprimarymyoblaststhroughactivationofMAPK/ERK1/2pathway.

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备注/Memo

备注/Memo:
收稿日期:2019-04-04.
基金项目:国家自然科学基金项目(31371356)、江苏省教育厅重大项目(13KJA180004).
通讯作者:陈华群,教授,研究方向:细胞生物学.E-mail:chenhuaqun@njnu.edu.cn
更新日期/Last Update: 2020-03-15