[1]苗向阳,王孝英,朱钦舒.基于金纳米颗粒聚集的高灵敏Ag+电致化学发光生物传感器[J].南京师范大学学报(自然科学版),2020,43(03):63-70.[doi:10.3969/j.issn.1001-4616.2020.03.011]
　Miao Xiangyang,Wang Xiaoying,Zhu Qinshu.Highly Sensitive Ag+ ECL Biosensor Based on Gold Nanoparticles Aggregation[J].Journal of Nanjing Normal University(Natural Science Edition),2020,43(03):63-70.[doi:10.3969/j.issn.1001-4616.2020.03.011]
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基于金纳米颗粒聚集的高灵敏Ag+电致化学发光生物传感器()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第43卷
期数:
2020年03期
页码:
63-70
栏目:
·化学·
出版日期:
2020-09-30

文章信息/Info

Title:
Highly Sensitive Ag+ ECL Biosensor Based on Gold Nanoparticles Aggregation
文章编号:
1001-4616(2020)03-0063-08
作者:
苗向阳1; 2; 王孝英1; 朱钦舒1
(1.南京师范大学分析测试中心,江苏 南京 210023)(2.苏州健雄职业技术学院医药科技学院,江苏 太仓 215411)
Author(s):
Miao Xiangyang1; 2; Wang Xiaoying1; Zhu Qinshu1
(1.Analytical and Testing Center,Nanjing Normal University,Nanjing 210023,China)(2.Department of Medical Science and Technology,Suzhou Chien-shiung Institute of Technology,Taicang 215411,China)
关键词:
C-Ag+-C; 金纳米颗粒; ECL; Ag+
Keywords:
C-Ag+-C; gold nanoparticle; ECL; Ag+
分类号:
O657.1 TP212.3
DOI:
10.3969/j.issn.1001-4616.2020.03.011
文献标志码:
A
摘要:
银离子是一种环境污染物,对其进行灵敏的检测非常重要. 本文构建了一种基于金纳米颗粒(AuNPs)聚集的高灵敏Ag+电致化学发光(ECL)生物传感器. 探针 DNA依靠Au-S键连接到金电极(GE)表面,用6-巯基-1-己醇(6-Hydroxy-1-hexanethiol,6-MCH)封闭未结合位点,合成尺寸均一的Au NPs,将其与Link DNA连接,通过与探针 DNA杂交连接到电极表面. 在杂交过程中加入一定浓度的Ag+孵育后,由于C-Ag+-C结构形成,诱导Au NPs大量聚集,提高电子传输效率. 最佳条件下,传感器检测Ag+浓度线性范围为0.5 nmol/L～10 μmol/L和10 μmol/L～500 μmol/L,最低检出限0.25 nmol/L,显示出较好的特异性和稳定性.
Abstract:
Silver ion is an environmental pollutant. It is very important to detect it sensitively. In this paper,a highly sensitive Ag+ ECL biosensor based on gold nanoparticle aggregation is constructed. First,the probe DNA is connected to the surface of the gold electrode(GE)by Au-S bond,and the unbound site is blocked by MCH. Subsequently,size-uniform Au NPs are synthesized and connected to the link DNA and then tied to the electrode surface by hybridization with probe DNA. When a certain concentration of Ag+ is added during the hybridization process,the stable C-Ag+-C structure is formed,which leads to aggregate Au NPs and accelerate the electron transport performance of the sensor. Under optimal conditions,the sensor is used as detector for the concentrations of Ag+. The linear ranges are obtained in the range of 0.5 nmol/L-10 μmol/L and 10 μmol/L-500 μmol/L. The detection limit of the ECL DNA biosensor is 0.25 nmol/L. This biosensor has good specificity and stability.

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备注/Memo

备注/Memo:
收稿日期:2020-04-21.
基金项目:国家自然科学基金项目(21974070)、太仓市基础研究计划项目(2018JC04).
通讯作者:朱钦舒,博士,高级实验师,研究方向:分析化学. E-mail:zhuqinshu@njnu.edu.cn
更新日期/Last Update: 2020-09-15