[1]刘倩梅,柯 敏,何馨媛,等.NCAPD3通过EZH2促进前列腺癌发生发展[J].南京师大学报(自然科学版),2021,44(04):53-59.[doi:10.3969/j.issn.1001-4616.2021.04.007]
　Liu Qianmei,Ke Min,He Xinyuan,et al.NCAPD3 Promotes the Occurrence and Development ofProstate Cancer Through EZH2[J].Journal of Nanjing Normal University(Natural Science Edition),2021,44(04):53-59.[doi:10.3969/j.issn.1001-4616.2021.04.007]
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NCAPD3通过EZH2促进前列腺癌发生发展()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第44卷
期数:
2021年04期
页码:
53-59
栏目:
·生物学·
出版日期:
2021-12-15

文章信息/Info

Title:
NCAPD3 Promotes the Occurrence and Development ofProstate Cancer Through EZH2
文章编号:
1001-4616(2021)04-0053-07
作者:
刘倩梅; 柯 敏; 何馨媛; 景作磊; 刘 平
南京师范大学生命科学学院,生物化学与生物制品研究所,江苏 南京 210023
Author(s):
Liu Qianmei; Ke Min; He Xinyuan; Jing Zuolei; Liu Ping
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Nanjing 210023,China
关键词:
前列腺癌; NCAPD3; EZH2; AR
Keywords:
Prostate cancer; NCAPD3; EZH2; AR
分类号:
R36
DOI:
10.3969/j.issn.1001-4616.2021.04.007
文献标志码:
A
摘要:
NCAPD3(non-SMC condensin II complex subunit D3)是凝缩蛋白复合物II的非SMC亚基之一,能够发挥调节染色质凝缩和分离的功能,在不同类型癌症中发挥促癌作用. 本研究探讨了NCAPD3通过调节EZH2(enhancer of zeste 2 polycomb repressive complex 2 subunit)蛋白的表达来促进前列腺癌的发生发展. 首先,通过数据库分析NCAPD3和EZH2在前列腺正常组织与肿瘤组织间的差异表达. 然后,选取前列腺癌细胞和临床组织样本为实验材料,通过Western blot、IHC等实验检测NCAPD3和EZH2在前列腺癌中的表达水平,发现NCAPD3和EZH2均在前列腺癌细胞及前列腺癌临床组织样本中高表达. 在过表达或敲低NCAPD3的细胞中,发现EZH2的蛋白和mRNA水平都相应地上升或下降,表明NCAPD3可以在转录水平调节EZH2的表达. 最后,过表达或敲低AR(androgen receptor),发现NCAPD3和EZH2蛋白水平相应地上升或下降,提示前列腺癌中存在AR-NCAPD3-EZH2信号通路.
Abstract:
NCAPD3(non-SMC condensin II complex subunit D3)is one of the non-SMC regulatory subunits of Condensin II,which is responsible for the condensation and segregation of chromosomes during mitosis and plays a role in promoting cancer in different cancers. This study explored that NCAPD3 promotes the occurrence and development of prostate cancer by regulating the expression of EZH2(enhancer of Zeste 2 polycomb repressive complex 2 subunit). Firstly,the differential expressions of NCAPD3 and EZH2 between normal and tumor tissues were analyzed through the database. Then,the levels of NCAPD3 and EZH2 in prostate cancer were detected by Western blot and IHC,and found that both NCAPD3 and EZH2 were highly expressed in prostate cancer cells and clinical tissues. After overexpressing or knocking down NCAPD3 in cells,the protein and mRNA levels of EZH2 increased or decreased accordingly,indicating that NCAPD3 regulates the expression of EZH2 at the transcriptional level. Finally,by overexpressing or knocking down AR(androgen receptor),it was found that the levels of NCAPD3 and EZH2 increased or decreased correspondingly,suggesting that there is an AR-NCAPD3-EZH2 signaling pathway in prostate cancer.

参考文献/References:

[1] AUCHUS R J,SHARIFI N. Sex hormones and prostate cancer[J]. Annual review of medicine,2020,71:33-45.
[2]SIEGEL R L,MILLER K D,JEMAL A. Cancer statistics,2019[J]. CA:cancer journal for clinicians,2019,69(1):7-34.
[3]CHEN W,ZHENG R,BAADE P D,et al. Cancer statistics in China,2015[J]. CA:journal for clinicians,2016,66(2):115-132.
[4]YEONG F M,HOMBAUER H,WENDT K S,et al. Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A[J]. Current biology,2003,13(23):2058-2064.
[5]GEORGE C M,BOZLER J,NGUYEN H Q,et al. Condensins are required for maintenance of nuclear architecture[J]. Cells,2014,3(3):865-882.
[6]LONGWORTH M S,HERR A,JI J Y,et al. RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3[J]. Genes & development,2008,22(8):1011-1024.
[7]HARTL T A,SWEENEY S J,KNEPLER P J,et al. Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis[J]. PLoS genetics,2008,4(10):e1000228.
[8]LAPOINTE J,MALHOTRA S,HIGGINS J P,et al. hCAP-D3 expression marks a prostate cancer subtype with favorable clinical behavior and androgen signaling signature[J]. American journal of surgical pathology,2008,32(2):205-209.
[9]KONDO Y,SHEN L,CHENG A S,et al. Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation[J]. Nature genetics,2008,40(6):741-750.
[10]VIRé E,BRENNER C,DEPLUS R,et al. The Polycomb group protein EZH2 directly controls DNA methylation[J]. Nature,2006,439(7078):871-874.
[11]MATSIKA A,SRINIVASAN B,DAY C,et al. Cancer stem cell markers in prostate cancer:an immunohistochemical study of ALDH1,SOX2 and EZH2[J]. Pathology,2015,47(7):622-628.
[12]SARAM?I O R,TAMMELA T L,MARTIKAINEN P M,et al. The gene for polycomb group protein enhancer of zeste homolog 2(EZH2)is amplified in late-stage prostate cancer[J]. Genes,chromosomes & cancer,2006,45(7):639-645.
[13]DEKKER J,MIRNY L. The 3D genome as moderator of chromosomal communication[J]. Cell,2016,164(6):1110-1121.
[14]FENG X D,SONG Q,LI C W,et al. Structural maintenance of chromosomes 4 is a predictor of survival and a novel therapeutic target in colorectal cancer[J]. Asian pacific journal of cancer prevention,2014,15(21):9459-9465.
[15]JE E M,YOO N J,LEE S H. Mutational and expressional analysis of SMC2 gene in gastric and colorectal cancers with microsatellite instability[J]. Apmis,2014,122(6):499-504.
[16]YIN L,JIANG L P,SHEN Q S,et al. NCAPH plays important roles in human colon cancer[J]. Cell death & disease,2017,8(3):e2680.

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备注/Memo

备注/Memo:
收稿日期:2021-03-18.
基金项目:国家自然科学基金项目(81472415、81872104).
通讯作者:刘平,博士,教授,研究方向:前列腺肿瘤发生发展和转移的分子基础. E-mail:liuping0805@njnu.edu.cn
更新日期/Last Update: 2021-12-15